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|Title:||Antioxidant properties of hydroxytyrosyl acetate compared with hydroxytyrosol and their protective capacity against oxidative stress in human neuroblastoma cells|
Giusti, Anna M
|Keywords:||Antioxidants;Hydroxytyrosol (HTy);Hydroxytyrosyl acetate (HTy-ac);Neuroblastoma cell line;Olive oil;Oxidative stress|
|Abstract:||This study analyses antioxidant activity of synthetic hydroxytyrosol (HTy) and hydroxytyrosyl acetate (HTy-ac) against different reactive species. Both phenols were found to be able to scavenge superoxide anion as well as hydroxyl radical with comparable efficacy (40% and 80% scavenging activity, respectively). HTy-ac was found to be more effective than HTy or a-tocopherol as hydrogen peroxide scavenger (53%, 29% and 25% scavenging activity, respectively) (p£0.05). Human neuroblastoma cell line SH-SY5Y was chosen as an ex vivo model and cellular oxidative stress was assessed through the measurement of total free sulfhydryl groups and protein carbonyls. HTy displayed a high antioxidant ability against thiols oxidation at 10 M, whereas HTy-ac showed a great capacity in preventing carbonyl groups generation. Different antioxidant ability showed by tested compounds against oxidative stress in biological system can be attributed to molecular concentration, reducing power and lipophilicity.|
|ISSN:||0975-1084 (Online); 0022-4456 (Print)|
|Appears in Collections:||JSIR Vol.70(11) [November 2011]|
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