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Title: Study on 16s rRNA based PCR method for specific detection of <i>Salmonella entrica typhi</i> from gut of infected silkworm <i>Bombyx mori (Linn.)</i>
Authors: Pradhap, M
Mathivanan, V
Parthasarathy, V
Ayyappan, J V Ananda
Kumar, S Sathees
Keywords: PCR
Primer annealing
Issue Date: Nov-2011
Publisher: NISCAIR-CSIR, India
Abstract: This study presents a novel molecular method for detection of <i>Salmonella entrica typhi </i>strain based on 16s rRNA sequence analysis by modification of PCR primer 16sF1, which was combined with a newly developed primer. Annealing method at the position of 50°C PCR amplification of 16s rRNA was performed with universal primer 27F and 1429R. Combination of these two specific primers proved specific detection of <i>Salmonella entrica typhi</i>, which was isolated and cultured from gut of silk worm <i>Bombyx mori </i>(Linn.).
Description: 909-911
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Appears in Collections:JSIR Vol.70(11) [November 2011]

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