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Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.70 [2011] >
JSIR Vol.70(11) [November 2011] >
| Title: | Study on 16s rRNA based PCR method for specific detection of Salmonella entrica typhi from gut of infected silkworm Bombyx mori (Linn.) |
| Authors: | Pradhap, M
Selvisabhanayakam
Mathivanan, V
Parthasarathy, V
Ayyappan, J V Ananda
Kumar, S Sathees |
| Keywords: | PCR
Primer
Primer annealing
Proteobacteria
Salmonella |
| Issue Date: | Nov-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | This
study presents a novel molecular method for detection of Salmonella
entrica typhi strain based on 16s rRNA sequence
analysis
by modification of PCR primer 16sF1, which was combined with a newly developed
primer. Annealing method at the
position
of 50°C PCR amplification of 16s rRNA was performed with universal primer 27F
and 1429R. Combination of these two
specific
primers proved specific detection of Salmonella entrica
typhi, which was isolated and cultured from gut of
silk worm
Bombyx mori (Linn.). |
| Page(s): | 909-911 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1084 (Online); 0022-4456 (Print) |
| Source: | JSIR Vol.70(11) [November 2011]
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