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NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.48 [2011] >
IJBB Vol.48(5) [October 2011] >
| Title: | Biosynthesis of gold nanoparticles by actinomycete Streptomyces viridogens strain HM10 |
| Authors: | Balagurunathan, R
Radhakrishnan, M
Rajendran, R Babu
Velmurugan, D |
| Keywords: | Actinomycetes
Streptomycetes
Nanoparticles
Gold
Antibacterial activity |
| Issue Date: | Oct-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Biosynthesis
of gold nanoparticles by Streptomycetes
from Himalayan Mountain was undertaken for the first
time. Out of 10 actinomycete strains tested, four strains (D10, HM10, ANS2 and
MSU) showed evidence for the intracellular biosynthesis of gold nanoparticles,
among which the strain HM10 showed high potency. Presence of spherical and rod
shaped gold nanoparticles in mycelium of the strain HM10 was determined by
transmission electron microscopy (TEM) and X-ray diffraction analysis. The average
particle size ranged from 18-20 nm. UV spectral analysis indicated that the
reduction of chloroauric acid (HAuCl4) occurred within 24 h of
reaction period. Further, the strain HM10 showed enhanced growth at 1 and 10 mM
concentration of HAuCl4. The gold nanoparticles synthesized by the
strain HM10 showed good antibacterial activity against S. aureus and E. coli in
well-diffusion method. The potential actinomycete HM10 strain was
phenotypically characterized and identified as Streptomyces viridogens (HM10). Thus, actinomycete strain HM10
reported in this study is a newly added source for the biosynthesis of gold
nanoparticles. |
| Page(s): | 331-335 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.48(5) [October 2011]
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