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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(10) [October 2011] >
| Title: | Novel substrate (algal protein) for cultivation of Rhodospirillum rubrum |
| Authors: | Vatsala, T M
Rekha, R
Srividhya, R |
| Keywords: | Algal protein substrate
Phototropic bacteria
Phycocyanobilin
Phycocyanin
Protease
Rhodospirillum rubrum |
| Issue Date: | Oct-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Rhodospirillum
rubrum was grown under light anaerobic conditions
with phycocyanin (C-pc) extracted from Spirulina
platensis as the sole source of carbon and nitrogen. When grown under these conditions cellular components like
lipids, carbohydrates, protein, carotenoids, bacteriochlorophyll were similar
to the one grown with malic acid and ammonium chloride. Growth of R. rubrum increased with increase in
concentration of C-pc (200 to 1000 mg/l). R.
rubrum also utilized C-pc under dark anaerobic condition. With both malic
acid and C-pc as carbon sources C-pc was consumed only after exhaustion of
malic acid under light anaerobic condition. No aberration of cell morphology
was seen under scanning electron microscope (SEM). R. rubrum utilized both phycocyanobilin and phycoprotein individually
as well as in combination. When grown with 1000 mg/l of phycoprotein 450 mg/l
of biomass was obtained, and with combination of phycocyanobilin (75 mg/l) and
phycoprotein (925 mg/l) 610 mg/l of biomass was obtained. Phycocyanobilin alone
did not inhibit the growth of R. rubrum.
Utilization of C-pc with protease like activity was observed in plate assay.
Protease like activity was also observed as zones around the colonies in plates
containing sterilized casein, gelatin and filter sterilized bovine serum
albumin. No amino acids were detected in the supernatant when analyzed with
ninhydrin. Extracellular protease like activity was highest when C-pc was used
as substrate (2.8 U/ml). Intracellular protease like activity was not detected
in cell free extracts. |
| Page(s): | 773-780 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(10) [October 2011]
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