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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(10) [October 2011] >
| Title: | Antioxidant and gastroprotective activities of Andrographis paniculata (Hempedu Bumi) in Sprague Dawley rats |
| Authors: | Wasman, S Q
Mahmood, A A
Chua, Lee Suan
Alshawsh, Mohammed A
Hamdan, S |
| Keywords: | Andrographis paniculata
Antioxidant
Ethanol
Histology
Peptic ulcer |
| Issue Date: | Oct-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Antioxidant and
gastroprotective activities of aqueous and ethanolic extract of Andrographis paniculata leaves in rats
have been reported. Sprague Dawley rats, 6 per group were used and rats in
groups 1 to 6 were pretreated with (0.25% w/v) carboxymethyl cellulose
(negative control, 5 ml/kg), 20 mg/kg omeprazole (positive control), (250 mg/kg
and 500 mg/kg) of aqueous leaf extracts (APLAE) and (250 and 500 mg/kg) of
ethanol leaf extracts (APLEE) respectively. Animals were orally administered
with 95% ethanol (5 ml/kg) 60 min after their pretreatments. Rats were
sacrificed 1 h after treatment and gastric contents were collected to measure pH and mucous weight. Stomach was
analyzed for gross and histological changes. Ulcer control group showed
extensive lesions of gastric mucosal layer, whereas rats pretreated with
omeprazole, 250 and 500 mg/kg of APLAE showed significant and dose dependent
reduction in gastric lesions with increased pH
and mucus content of stomach. Rats pretreated with 250 or 500 mg/kg of APLEE
showed significantly better inhibition of gastric mucosal lesions. Further, the
in vitro antioxidant studies using
2,2-diphenyl-1-picrylhydrazyl (DPPH) assay showed that ethanol extracts have
superior free radical scavenging activity with IC50 value = 10.9
than aqueous extracts with
IC50 value = 24.65. Results of this study showed that pretreatment
with ethonolic extract of A. paniculata
ethanolic provided significant protection against gastric ulcer by regulating of pH,
mucous production and antioxidant property. |
| Page(s): | 767-772 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(10) [October 2011]
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