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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Natural Products and Resources (IJNPR)
[Formerly Natural Product Radiance (NPR)] >
IJNPR Vol.2 [2011] >
IJNPR Vol.2(3) [September 2011] >
| Title: | In vitro cytotoxicity of Bryonia laciniosa (Linn.) Naud. on human cancer cell lines |
| Authors: | Moghe, Alpana S
Gangal, Sudha G
Shilkar, Priya R |
| Keywords: | Bryonia laciniosa
Human cancer
Anticancer
Cytotoxicity
In vitro |
| Issue Date: | Sep-2011 |
| Publisher: | NISCAIR-CSIR, India |
| IPC Code: | A61K 36/00 |
| Abstract: | Screening and isolation of active components
from the herbs possessing anticancer potential appears to be a promising way of
discovering novel chemotherapeutic compounds. A traditional medicinal herb Bryonia laciniosa (Linn.) Naud. is an important source of
biologically active compounds used in Homoeopathy and has potential for further
exploratory medicinal use, especially for its anticancer properties. In the
present study water, methanol and chloroform extracts of B. laciniosa leaves were tested on human cancer and normal cell
lines using three in vitro
cytotoxicity assays, cell viability, SRB and clonogenic potential. The effect
was compared with that of standard anticancer drugs doxorubicin and
vincristine. Activation of caspase-8 and caspase-3 enzymes were assessed to
evaluate the effect of extract on induction of apoptosis in cells. Of the
different extracts, the aqueous extract demonstrated maximum cytotoxicity to
cancer cells. The IC50 value was estimated to be 18 µg/mL. Much
higher concentration (85 µg/mL) of the extract was required to produce same
effect on the normal cells. Nearly all cancer cells could be killed by the leaf
extracts of Bryonia in vitro, where as small fraction of
cells from cancer cell lines showed resistance to doxorubicin even at
concentration much higher than IC50. Results of caspase assay
demonstrated activation of both caspase-8 and caspase-3 enzymes indicating induction
of apoptosis in Bryonia leaf extract
treated cells. The results thus show that aqueous extract of B. laciniosa leaves possess cytotoxicity
to cancer cells and are able to kill all cancer cells without leaving residual
population. The extract also shows a better ability to discriminate between
cancer cells and normal cells as compared with standard drug using three in vitro assays. |
| Page(s): | 322-329 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1033 (Online); 0379-5136 (Print) |
| Source: | IJNPR Vol.2(3) [September 2011]
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