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Title: In vitro cytotoxicity of Bryonia laciniosa (Linn.) Naud. on human cancer cell lines
Authors: Moghe, Alpana S
Gangal, Sudha G
Shilkar, Priya R
Keywords: Bryonia laciniosa;Human cancer;Anticancer;Cytotoxicity;In vitro
Issue Date: Sep-2011
Publisher: NISCAIR-CSIR, India
IPC Code: A61K 36/00
Abstract: Screening and isolation of active components from the herbs possessing anticancer potential appears to be a promising way of discovering novel chemotherapeutic compounds. A traditional medicinal herb Bryonia laciniosa (Linn.) Naud. is an important source of biologically active compounds used in Homoeopathy and has potential for further exploratory medicinal use, especially for its anticancer properties. In the present study water, methanol and chloroform extracts of B. laciniosa leaves were tested on human cancer and normal cell lines using three in vitro cytotoxicity assays, cell viability, SRB and clonogenic potential. The effect was compared with that of standard anticancer drugs doxorubicin and vincristine. Activation of caspase-8 and caspase-3 enzymes were assessed to evaluate the effect of extract on induction of apoptosis in cells. Of the different extracts, the aqueous extract demonstrated maximum cytotoxicity to cancer cells. The IC50 value was estimated to be 18 µg/mL. Much higher concentration (85 µg/mL) of the extract was required to produce same effect on the normal cells. Nearly all cancer cells could be killed by the leaf extracts of Bryonia in vitro, where as small fraction of cells from cancer cell lines showed resistance to doxorubicin even at concentration much higher than IC50. Results of caspase assay demonstrated activation of both caspase-8 and caspase-3 enzymes indicating induction of apoptosis in Bryonia leaf extract treated cells. The results thus show that aqueous extract of B. laciniosa leaves possess cytotoxicity to cancer cells and are able to kill all cancer cells without leaving residual population. The extract also shows a better ability to discriminate between cancer cells and normal cells as compared with standard drug using three in vitro assays.
Page(s): 322-329
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJNPR Vol.2(3) [September 2011]

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