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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.48 [2011] >
IJBB Vol.48(4) [August 2011] >
| Title: | Preferential recognition of methylglyoxal-modified calf thymus DNA by circulating antibodies in cancer patients |
| Authors: | Ahmad, M Imtiaz
Ahmad, Saheem
Moinuddin |
| Keywords: | Methylglyoxal
Cancer
Calf thymus DNA
Autoimmunity |
| Issue Date: | Aug-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Methylglyoxal (MG) has been implicated in
mutagenesis and cancer. Present study probes the antigenicity of MG damaged DNA
in cancer patients. Purified calf thymus DNA was damaged by the synergistic
action of MG, lysine (Lys) and CuSO4 for
24 h at 37oC. DNA modifications produced single-strand breaks,
hyperchromicity in UV spectrum and increased fluorescence intensity. Binding
characteristics of auto-antibodies in cancer patients were assessed by direct
binding and inhibition ELISA. These antibodies exhibited enhanced binding with
the modified DNA, as compared to the native form. The effect was more
pronounced when affinity-purified IgG was used in place of the serum. In
conclusion, MG-modified DNA presents unique epitopes which are recognized as non-self by the immune system and may,
therefore, be one of the factors for the autoantibody induction in cancer
patients. |
| Page(s): | 290-296 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.48(4) [August 2011]
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