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dc.contributor.authorNaushad, Shaik Mohammad-
dc.contributor.authorPavani, Addepalli-
dc.contributor.authorRupasree, Yedluri-
dc.contributor.authorSripurna, Deepti-
dc.contributor.authorGottumukkala, Suryanarayana Raju-
dc.contributor.authorDigumarti, Raghunadha Rao-
dc.contributor.authorKutala, Vijay Kumar-
dc.identifier.issn0975-0959 (Online); 0301-1208 (Print)-
dc.description.abstractThe present study was aimed to investigate the modulatory role of plasma folate and eight putatively functional polymorphisms of one-carbon metabolism on catecholamine methyltransferase (COMT)-mediated oxidative DNA damage and breast cancer risk. Plasma folate and 8-oxo-2’-deoxyguanosine (8-oxodG) were estimated by commercially available kits, while polymorphisms were screened by PCR-RFLP and PCR-AFLP methods. COMT H108L polymorphism showed independent association with breast cancer (OR: 1.73, 95% CI: 1.31-2.30). No significant interaction was observed between folate status and COMT genotype. Multifactor dimensionality reduction (MDR) analysis gave evidence for the significant epistatic (gene-gene) interactions (p<0.0001) of COMT H108L with reduced folate carrier 1 (RFC1) G80A, thymidylate synthase (TYMS) 5’-UTR 3R2R, TYMS 3’-UTR ins6/del6. Increased plasma 8-oxodG were observed in cases compared to controls (mean ± SE: 5.59 ± 0.60 vs. 3.50 ± 0.40 ng/ml, p<0.004). Plasma folate deficiency alone was not a significant predictor of 8-oxodG elevation. The genotype combinations namely, RFC1 G80A/methionine synthase reductase (MTRR) A66G, RFC1 G80A/SHMT C1420T/TYMS 3R2R and serine hydroxymethyltransferase (SHMT) C1420T/TYMS 3R2R/methionine synthase (MTR) A2756G/COMT H108L were strong predictors of 8-oxodG elevation in the order of risk. To conclude, the current study provides substantial evidence for a cross talk between one-carbon metabolism and COMT catalysis that might influence oxidative DNA damage and breast cancer risk.en_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.rights CC Attribution-Noncommercial-No Derivative Works 2.5 Indiaen_US
dc.sourceIJBB Vol.48(4) [August 2011]en_US
dc.subjectCatechol-O-methyltransferase (COMT)en_US
dc.subject8-Oxo-2’-deoxyguanosine (8-oxodG)en_US
dc.subjectOne-carbon metabolismen_US
dc.subjectOxidative DNA damageen_US
dc.subjectBreast canceren_US
dc.subjectPlasma folateen_US
dc.titleModulatory effect of plasma folate and polymorphisms in one-carbon metabolism on catecholamine methyltransferase (COMT) H108L associated oxidative DNA damage and breast cancer risken_US
Appears in Collections:IJBB Vol.48(4) [August 2011]

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