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Title: <i>In vitro</i> propagation and mass scale multiplication of a critically endangered epiphytic orchid, <i style="">Gastrochilus calceolaris </i>(Buch.-Ham ex J.E.Sm.) D.Don.
Authors: Pathak, Promila
Piri, Hossein
Vij, S P
Mahant, K C
Chauhan, Shaveta
Keywords: <i style="">Gastrochilus calceolaris</i>
Immature seeds
Nutrient media
Seed germination
Seedling development
Issue Date: Sep-2011
Publisher: NISCAIR-CSIR, India
Abstract: <i>In vitro</i> asymbiotic seed germination potential of its immature seeds (36 weeks after pollination) of <i>G.</i><i style=""> calceolaris </i>was successfully tested on three different agar gelled nutrient media i.e. Murashige and Skoog (MS), Mitra <i style="">et al. </i>(M) and potato dextrose agar (PDA). Seeds germinated within 15.75±0.75 to 35.75±0.75 days in the three different media. The protocorms developed therefrom subsequently differentiated into first leaf and root primordia, and complete seedlings were obtained within 111.25±1.25 to 141.25±1.25 days on MS and M media. The protocorms, though failed to differentiate further on basal PDA medium, despite repeated subculturings, incorporation of peptone (P; 1gl<sup>-1</sup>), yeast extract (YE; 2 gl<sup>-1</sup>) and coconut water (CW; 20%) in the medium proved beneficial in inducing differentiation, in these germinating entities. Additional use of growth additives (P/YE/CW), in general, favoured better germination, protocorm formation and seedling development. The optimal nutritional combination during seed germination, protocorm growth and multiplication and seedling development was found to be CW (10%) enriched MS medium.
Description: 711-716
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.49(09) [September 2011]

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