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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(09) [September 2011] >
| Title: | In vitro propagation and mass scale multiplication of a critically endangered epiphytic orchid, Gastrochilus calceolaris (Buch.-Ham ex J.E.Sm.) D.Don. |
| Authors: | Pathak, Promila
Piri, Hossein
Vij, S P
Mahant, K C
Chauhan, Shaveta |
| Keywords: | Gastrochilus calceolaris
Immature seeds
Nutrient media
Seed germination
Seedling development |
| Issue Date: | Sep-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In vitro asymbiotic seed germination potential of its immature seeds (36 weeks after pollination) of G. calceolaris was successfully tested on three different agar gelled
nutrient media i.e. Murashige and Skoog (MS), Mitra et al. (M) and potato dextrose agar (PDA).
Seeds germinated within 15.75±0.75 to 35.75±0.75 days in the three
different media. The protocorms developed therefrom subsequently differentiated
into first leaf and root primordia, and complete seedlings were obtained within
111.25±1.25 to 141.25±1.25 days on MS and M media. The protocorms, though
failed to differentiate further on basal PDA medium, despite repeated
subculturings, incorporation of
peptone (P; 1gl-1), yeast extract (YE; 2 gl-1) and
coconut water (CW; 20%) in the medium proved beneficial in inducing
differentiation, in these germinating entities. Additional use of growth
additives (P/YE/CW), in general, favoured better germination, protocorm
formation and seedling development. The optimal nutritional combination during
seed germination, protocorm growth and multiplication and seedling development
was found to be CW (10%) enriched MS medium. |
| Page(s): | 711-716 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(09) [September 2011]
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