Effect of green tea (<i style="">Camellia sinensis</i> L.) extract on morphological and functional changes in adult male gonads of albino rats

Abstract

Green tea, prepared from the steamed and dried leaves of the shrub <i style="">Camellia sinensis,</i> is known for its antioxidant and anti-carcinogenic effects. However, its effects on male gonadal functions have not been explored adequately and the present investigation has been undertaken to evaluate the effect of green tea extract on gonads of adult male albino rats. Results of <i style="">in vivo</i> studies showed that green tea extract (GTE) at mild (1.25 g%, ≡ 5 cups of tea/day), moderate (2.5 g%, ≡ 10 cups of tea/day) and high (5.0 g%, ≡ 20 cups of tea/day) doses, for a period of 26 days, altered morphology and histology of testis and accessory sex organs. A significant dose-dependent decrease in the sperm counts, inhibited activities of testicular ∆⁵3<img src='/image/spc_char/beta.gif' border=0>- and 17<img src='/image/spc_char/beta.gif' border=0>-hydroxysteroid dehydrogenase (∆⁵-3<img src='/image/spc_char/beta.gif' border=0>-HSD and 17<img src='/image/spc_char/beta.gif' border=0>-HSD respectively) and decreased serum testosterone level were noticed. Significant increase in serum LH level was observed after moderate and high doses; serum FSH level also increased but not significantly. Histopathological examination showed inhibition of spermatogenesis evidenced by preferential loss of matured and elongated spermatids. Results of this study showed that GTE at relatively high dose may cause impairment of both the morphological and normal functional status of testis in rodents and thus its consumption at relatively high doses raises concern on male reproductive function in spite of its other beneficial effects.