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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(09) [September 2011] >
| Title: | Involvement of AMPA receptors for Mesobuthus tamulus Pocock venom-induced depression of monosynaptic reflex in neonatal rat spinal cord in vitro |
| Authors: | Maurya, Amar N
Deshpande, Shripad B |
| Keywords: | CNQX
Indian red scorpion venom
Non-NMDA receptors
Spinal synaptic transmission |
| Issue Date: | Sep-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Glutamate is a putative neurotransmitter at
Ia-α motoneuron synapse in the spinal cord and mediate the action via
N-methyl-D-aspartate (NMDA) and
α-amino-3-hydroxy-5-methyl-4-isoxazole-propionate (AMPA) receptors. Since NMDA
receptors are not involved in M. tamulus
Pocock (MBT) venom-induced depression of spinal monosynaptic reflex (MSR), the
present study was undertaken to evaluate the role of AMPA receptors in
mediating the depression of MSR by MBT venom. The experiments were performed on
isolated hemisected spinal cord from 4-6 day old rats. Stimulation of a dorsal
root with supramaximal voltage evoked MSR and polysynaptic reflex (PSR)
potentials in the corresponding segmental ventral root. Superfusion of MBT
venom (0.3 µg/ml) depressed the spinal reflexes in a time-dependent manner. The
maximum depression of MSR(~ 66%) was seen at 10 min and it was 25 min for PSR (~ 75%). The time to
produce 50% depression of MSR and PSR was 6.7 ± 1.5 and 10.8 ± 2.6 min,
respectively. Pretreatment of the cords with 6-cyano-7-nitroquinoxaline-2,
3-dione (CNQX, 0.1 μM), an AMPA receptor antagonist, blocked the
venom-induced depression of MSR but not PSR. The results indicate that
venom-induced depression of MSR is mediated via AMPA receptors. |
| Page(s): | 660-663 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(09) [September 2011]
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