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JSIR Vol.66 [2007] >
JSIR Vol.66(05) [May 2007] >
| Title: | Characterization of an actinomycete isolated from the estuarine finfish, Mugil cephalus Lin. (1758) and its optimization for cellulase production |
| Authors: | Murugan, M
Srinivasan, M
Sivakumar, K
Sahu, Maloy Kumar
Kannan, L |
| Keywords: | CMC-Cellulase
Mugil cephalus
SDS-PAGE
Streptomyces actuosus |
| Issue Date: | May-2007 |
| Publisher: | CSIR |
| Abstract: | Actinomycetes (35 strains), isolated from the gut of estuarine finfish Mugil cephalus collected from the Vellar
estuary, were examined for cellulase activity. Strain CL-30, tentatively identified as Streptomyces actuosus, showed maximum
cellulase activity at: pH 7, temperature 35°C, NaCl concentration 1-2%, carbon source sucrose and without addition of any
amino acids. Molecular weight of cellulase determined by SDS-PAGE was 110 kDa. The study indicates the scope for the use
of S. actuosus as an ideal organism for the industrial production of extracellular cellulase enzyme. |
| Page(s): | 388-393 |
| ISSN: | 0022-4456 |
| Source: | JSIR Vol.66(05) [May 2007]
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