22-May-2013
18:58:11 IST
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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(09) [September 2011] >
| Title: | Characterization and in vitro expression of non-structural 1 protein of canine parvovirus (CPV-2) in mammalian cell line |
| Authors: | Saxena, Lovleen
Chaturvedi, Uttara
Saxena, Shikha
Kumar, G Ravi
Sahoo, A P
Kumar, Sudesh
Doley, J
Rajmani, R S
Singh, Prafull K
Kumar, Rajiv
Tiwari, Ashok K |
| Keywords: | Eukaryotic expression vector
Canine parvovirus
NS-1 gene
Non-structural protein 1
Polymerase chain reaction |
| Issue Date: | Sep-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Parvoviruses are small, 260-Å-diameter,
icosahedral, non-enveloped, single-stranded DNA viruses with a genome of
approximately 5 kb. Non structural protein, (NS-1) is especially relevant,
being both essential for virus replication and the main factor responsible for
virus pathogenicity and cytotoxicity. This protein has also been reported to
possess the property of killing of transformed cells. The present study was
carried out to clone, characterize and express the NS-1 gene of canine
parvovirus. NS-1 complete CDS 2020bp was amplified, cloned into eukaryotic expression
vector pcDNA 3.1(+), sequenced and characterized by in vitro expression analysis. Functional activity of recombinant
construct, pcDNA.cpv.NS-1, was evaluated by RT-PCR and flow cytometry for the
expression of NS-1 specific mRNA and NS-1 protein, respectively, in transfected
HeLa cells. This recombinant plasmid may serve as an important tool to evaluate
the apoptotic potential of NS-1 protein of canine parvovirus in cultured HeLa
cells. |
| Page(s): | 654-659 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(09) [September 2011]
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