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Title: Protoplast formation and regeneration of Acetobacter pasteurianum NCIM 2314
Authors: Singhvi, Mamta
Joshi, Dipti
Gokhale, D V
Issue Date: Jun-2011
Publisher: NISCAIR-CSIR, India
Abstract: Method for production and regeneration of Acetobacter pasteurianum NCIM 2314 protoplasts are described. The protoplasts were obtained by treatment with lysozyme in protoplast buffer at pH 8.0 with different osmotic stabilizers. The protoplasts were regenerated on Acetobacter medium with various osmotic stabilizers. Maximum protoplasts formation was obtained in protoplasts buffer with NaC1 as an osmotic stabilizer using lysozyme (0.5mg/mL). Maximum protoplast regeneation was obtained on Acetobacter medium with NaCl (0.5M) as an osmotic stabilizer. The regeneration medium was also applicable to other species of Acetobactor as well. This is, to our knowledge, the first report on protoplast formation and efficient regeneration in case of Acetobacter pasteurianum.
Page(s): 94-96
ISSN: 0975-2412 (Online); 0771-7706 (Print)
Appears in Collections:BVAAP Vol.19(1) [June 2011]

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