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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(08) [August 2011] >
| Title: | Expression of rabies virus glycoprotein gene into eukaryotic system and determination of potential T-cell epitopes |
| Authors: | Tomar, Neha R
Chandra, Rajesh
kumar, Rajiv
Tiwari, A K
Kumar, Anil |
| Keywords: | DNA vaccine
Epitope mapping
Glycoprotein
Indirect fluorescent antibody test
Rabies virus |
| Issue Date: | Aug-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | The present
study was undertaken to clone, express rabies virus glycoprotein (RVG) and to
identify potential T-cell epitopes on it. RVG gene (1590 bp) was amplified
using gene specific primers. The amplified product was cloned into pTZ57R/T
cloning vector by TA cloning. RVG gene was subcloned into pcDNA3.1 (+)
expression vector. In this study, cloning and expression of rabies virus
glycoprotein gene was done under CMV promoter and an expression construct
(pcDNA.RVG) was prepared and clones were confirmed by restriction digestion,
colony PCR and nucleotide sequencing. The expression construct was further
characterized by western blotting and indirect fluorescent antibody test
(IFAT).
In silico analysis of this protein was done to find out potential antigenic
sites so that it can be further evaluated for its potential as candidate for
epitope vaccine against rabies. |
| Page(s): | 594-599 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(08) [August 2011]
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