NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(08) [August 2011] >

Title: Effects of -arrestin 2 on cytokine production of CD4+ T lymphocytes of mice with allergic asthma
Authors: Wang, Guyi
Liu, Yi
Yang, Muyi
Liu, Shaokun
Ma, Libing
Gong, Subo
Li, Keng
Zhang, Li
Xiang, Xudong
Keywords: Asthma
-arrestin 2
2 adrenergic receptors
CD4+ T lymphocytes
Issue Date: Aug-2011
Publisher: NISCAIR-CSIR, India
Abstract: -arrestin 2 has been shown to participate in the pathogenesis of asthma by inducing Th2 cell migration to the lungs. Whether -arrestin 2 regulates cytokine production of CD4+ T cells is still unknown. The aim of the present study was to investigate the effect of -arrestin 2 on the cytokine production of CD4+ T lymphocytes and the mechanism involved in a mouse model for asthma. After silencing -arrestin 2 expression in CD4+ T lymphocytes from asthmatic mice by RNA interference (RNAi), the interleukin-4 (IL-4) and interferon- (IFN-) levels in CD4+ T lymphocyte culture supernatants with or without terbutaline stimulation were determined. Cell-surface 2 adrenergic receptor (2AR) as well as GATA3 expression of CD4+ T lymphocytes were also measured. CD4+ T lymphocytes of mice with allergic asthma expressed higher levels of -arrestin 2 on both mRNA and protein levels. -arrestin 2 RNAi decreased IL-4 (43.16%) and GATA3 (protein 77.21%, mRNA 62.98%) expression after terbutaline stimulation. Cell-surface 2AR of CD4+ T lymphocytes decreased (15.27%) after terbutaline treatment, but recovered after -arrestin 2 RNAi down-modulation. These findings demonstrate that -arrestin 2 regulates IL-4 production and GATA3 expression of CD4+ T lymphocytes partly through the 2AR signaling pathway in an allergic asthma model.
Page(s): 585-593
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Source:IJEB Vol.49(08) [August 2011]

Files in This Item:

File Description SizeFormat
IJEB 49(8) 585-593.pdf292.45 kBAdobe PDFView/Open
 Current Page Visits: 113 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 135762 since 01-Sep-2015  Last updated on 27-May-2016Webmaster: