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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(08) [August 2011] >
| Title: | Effects of  -arrestin 2 on cytokine production of CD4+ T lymphocytes of mice with allergic asthma |
| Authors: | Wang, Guyi
Liu, Yi
Yang, Muyi
Liu, Shaokun
Ma, Libing
Gong, Subo
Li, Keng
Zhang, Li
Xiang, Xudong |
| Keywords: | Asthma
-arrestin 2
2 adrenergic receptors
CD4+ T lymphocytes
Interleukin-4 |
| Issue Date: | Aug-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | -arrestin 2 has been shown to participate in the pathogenesis of asthma by inducing Th2 cell migration to the lungs. Whether -arrestin 2 regulates cytokine production of CD4+ T cells is still unknown. The aim of the present study was to investigate the effect of -arrestin 2 on the cytokine production of CD4+ T lymphocytes and the mechanism involved in a mouse model for asthma. After silencing -arrestin 2 expression in CD4+ T lymphocytes from asthmatic mice by RNA interference (RNAi), the interleukin-4 (IL-4) and interferon- (IFN-) levels in CD4+ T lymphocyte culture supernatants with or without terbutaline stimulation were determined. Cell-surface 2 adrenergic receptor (2AR) as well as GATA3 expression of CD4+ T lymphocytes were also measured. CD4+ T lymphocytes of mice with allergic asthma expressed higher levels of -arrestin 2 on both mRNA and protein levels. -arrestin 2 RNAi decreased IL-4 (43.16%) and GATA3 (protein 77.21%, mRNA 62.98%) expression after terbutaline stimulation. Cell-surface 2AR of CD4+ T lymphocytes decreased (15.27%) after terbutaline treatment, but recovered after -arrestin 2 RNAi down-modulation. These findings demonstrate that -arrestin 2 regulates IL-4 production and GATA3 expression of CD4+ T lymphocytes partly through the 2AR signaling pathway in an allergic asthma model. |
| Page(s): | 585-593 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(08) [August 2011]
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