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Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.66 [2007] >
JSIR Vol.66(05) [May 2007] >
| Title: | Ion pairing RP-HPLC analytical methods for simultaneous estimation of simvastatin and its â-hydroxy acid |
| Authors: | Madan, Jitender
Thakkar, Vikrant
Dwivedi, Anil Kumar
Singh, Satyawan |
| Keywords: | HPLC assay method
Hypolipidemic agent
Simvastatin |
| Issue Date: | May-2007 |
| Publisher: | CSIR |
| IPC Code: | A61K |
| Abstract: | Sensitive HPLC assay methods for Simvastatin (SV), a hypolipidemic agent, and its corresponding â-hydroxy
acid (SVA), were developed and validated for their simultaneous estimation in solutions of various studies. HPLC separations
were achieved on (i) C₈ Lichrocart® Lichrosphere® (ii) CN Lichrocart® Lichrosphere® and (iii) C₁₈ Phenomenex® columns.
The eluents were monitored by diode array detector at 240 nm. Retention times were: SV, 8-9; and SVA, 5.5-6 min. The
lower limit of detection of both SV and SVA on C₁₈ column was 0.05 μg/ml and on C₈ and CN columns was 0.1 μg/ml.
Reproducibility of the method were determined by inter and intra assay precision (< 6%). |
| Page(s): | 371-376 |
| ISSN: | 0022-4456 |
| Source: | JSIR Vol.66(05) [May 2007]
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