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Title: Isolation, characterization and growth response of biofilm forming bacteria Bacillus pumilus from the sea grass, Halodule pinifolia off Kanyakumari coast
Authors: Merina, Medo
Lipton, A.P.
Wesley, S. Godwin
Keywords: Bacteria;Biofilm;Colony forming units;Sea grass extract;Growth response
Issue Date: Jun-2011
Publisher: NISCAIR-CSIR, India
Abstract: Bacteria from the biofilms of the blade of sea grass, Halodule pinifolia occurring along the sea grass beds off Kanyakumari coast (Tamil Nadu) were isolated. Bacteria identified included Pseudomonas, Staphylococcus, E.coli and Bacillus species. Predominant biofilm bacterium was characterized as Bacillus pumilus (Accession No. HM006706) by standard biochemical and molecular methods. Sea grass biofilm associated B. pumilus strain was aerobic, Gram positive, rod-shaped, spore-forming motile bacteria. The 16s rDNA gene sequence of the new isolate B. pumilus had shown maximum similarity (96%) with other B. pumilus strains PRE14, NHIC3, CT3. Growth response due to interaction of B. pumilus in the presence of sea grass extract was evaluated by inoculating in different concentration of sea grass extract. Results indicated that more colony forming units (4.6×1011 CFUs) in Zobell marine broth supplemented with 20.0% of the water extract of Halodule pinifolia.
Page(s): 443-448
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.40(3) [June 2011]

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