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Title: Isolation, characterization and growth response of biofilm forming bacteria <i style="">Bacillus pumilus</i> from the sea grass, <i>Halodule pinifolia</i> off Kanyakumari coast
Authors: Merina, Medo
Lipton, A.P.
Wesley, S. Godwin
Keywords: Bacteria
Colony forming units
Sea grass extract
Growth response
Issue Date: Jun-2011
Publisher: NISCAIR-CSIR, India
Abstract: Bacteria from the biofilms of the blade of sea grass, <i>Halodule pinifolia</i> occurring along the sea grass beds off Kanyakumari coast (Tamil Nadu) were isolated. Bacteria identified included <i style="">Pseudomonas, Staphylococcus, E.coli</i> and <i style="">Bacillus</i> species. Predominant biofilm bacterium was characterized as<i> Bacillus pumilus</i> (Accession No. HM006706) by standard biochemical and molecular methods. Sea grass biofilm associated <i>B. pumilus</i> strain was aerobic, Gram positive, rod-shaped, spore-forming motile bacteria. The 16s rDNA gene sequence of the new isolate <i>B. pumilus</i> had shown maximum similarity (96%) with other <i style="">B. pumilus</i> strains PRE14, NHIC3, CT3. Growth response due to interaction of <i>B. pumilus</i> in the presence of sea grass extract was evaluated by inoculating in different concentration of sea grass extract. Results indicated that more colony forming units (4.6×10<sup>11</sup> CFUs) in Zobell marine broth supplemented with 20.0% of the water extract of <i>Halodule pinifolia</i>.
Description: 443-448
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.40(3) [June 2011]

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