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Title: Diversity of Bacillus and Actinomycetes in the water and sediment samples from Kumarakom region of Vembanadu lake
Authors: George, Maya
Cyriac, Neethu
Nair, Aswathi
M, Hatha A A
Keywords: Wetlands;Microbial Diversity;Bacillus;Actinomycetes;Hydrolytic enzymes;Antibacterial activity
Issue Date: Jun-2011
Publisher: NISCAIR-CSIR, India
Abstract: Diversity of different groups of Bacillus and Actinomycetes in the water and sediment samples from Kumarakom estuary was analyzed to find out potential strains for further application. Bacillus genera were identified and grouped into five phenogroups such as Bacillus polymyxa, Bacillus subtilis, Bacillus sphaericus, Thermophiles and Alicyclobacillus. Phenogroups show differences in the shape of the spore (oval or spherical), position of the spore (central or terminal) and swelling of the sporangium. Ability of the isolates to elaborate various hydrolytic enzymes and their ability to reduce nitrate and ferment various carbohydrate sources were also studied. Different plating media such as glycerol arginine agar, starch-casein agar and soil extract agar were used to isolate the Actinomycetes. Glycerol arginine agar found to be superior to the other two plating media. Diversity of Actinomycetes was less when compared to that of Bacillus genera. Potential of the Actinomycetes to produce antibiotics were evaluated by well diffusion method against 6 serotypes of Salmonella, Vibrio parahaemolyticus and Escherichia coli.
Page(s): 430-437
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJMS Vol.40(3) [June 2011]

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