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JSIR Vol.66 [2007] >
JSIR Vol.66(03) [March 2007] >
| Title: | Characterization of Hela cells for presentation of exogenous antigens |
| Authors: | Devasahayam, Mercy |
| Keywords: | Core antigen
Endocytosis
Hela
Hepatitis B virus
MHC |
| Issue Date: | Mar-2007 |
| Publisher: | CSIR |
| Abstract: | Role of MHC class I HLA A2 HC and trimeric molecules on the binding of exogenous HBV core antigen was studied
in Hela cells transfected with MHC class 1 HLA A2. HC and trimeric HLA A2 molecules could be identified with specific
monoclonal antibodies. HC and trimeric HLA-A2 molecules exist in varying proportion on the cell surface of Hela cells.
MHC class I HC molecules on the cell surface do not bind HBV core peptide. The exchange of peptide with MHC class 1
molecules on the cell surface is inefficient with cells exhibiting a maximal binding (12%) at 1.5μM of peptide. Hence,
presentation of exogenous HBV core antigen molecules occurs after endocytosis of the antigen and MHC class 1 molecules,
followed by functional trimer complex formation in endolysosomal compartments. |
| Page(s): | 243-249 |
| ISSN: | 0022-4456 |
| Source: | JSIR Vol.66(03) [March 2007]
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