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Title: Characterization of Hela cells for presentation of exogenous antigens
Authors: Devasahayam, Mercy
Keywords: Core antigen;Endocytosis;Hela;Hepatitis B virus;MHC
Issue Date: Mar-2007
Publisher: CSIR
Abstract: Role of MHC class I HLA A2 HC and trimeric molecules on the binding of exogenous HBV core antigen was studied in Hela cells transfected with MHC class 1 HLA A2. HC and trimeric HLA A2 molecules could be identified with specific monoclonal antibodies. HC and trimeric HLA-A2 molecules exist in varying proportion on the cell surface of Hela cells. MHC class I HC molecules on the cell surface do not bind HBV core peptide. The exchange of peptide with MHC class 1 molecules on the cell surface is inefficient with cells exhibiting a maximal binding (12%) at 1.5μM of peptide. Hence, presentation of exogenous HBV core antigen molecules occurs after endocytosis of the antigen and MHC class 1 molecules, followed by functional trimer complex formation in endolysosomal compartments.
Page(s): 243-249
ISSN: 0022-4456
Appears in Collections:JSIR Vol.66(03) [March 2007]

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