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IJBB Vol.44(4) [August 2007] >


Title: Purification and properties of an extracellular pectin lyase produced by the strain of Penicillium oxalicum in solid-state fermentation
Authors: Yadav, Sangeeta
Shastri, N V
Keywords: Pectins
Pectin lyases
Pectin transeliminases
Penicillium oxalicum
Solid-state fermentation
Issue Date: Aug-2007
Publisher: CSIR
Abstract: A pectin lyase (PNL, EC 4.2.2.10) produced extracellularly by the strain of Penicillium oxalicum in solid-state fermentation medium containing deoiled mandarin orange peel meal was purified to apparent homogeneity by a protocol that included ammonium sulfate precipitation, DEAE-Sephadex A-50 and Sephadex G-100 chromatography. The enzyme had molecular mass of 50 kD, as determined by SDS- PAGE and showed optimum pH and temperature at 8.0 and 50ºC respectively. It had an isoelectric point (pI) of 5.0 and showed a Km of 1.1 mg/ml of citrus pectin. The enzyme was strongly inhibited by Mo⁴⁺, Ag⁺ and Pb²⁺ and also by polyphenolic compounds, in particular tannic acid.
Page(s): 247-251
ISSN: 0301-1208
Source:IJBB Vol.44(4) [August 2007]

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