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Title: Indirect organogenesis of Vitex trifolia Linn.-An important medicinal plant
Authors: Arulanandam L, John Peter
Ghanthikumar, S
Keywords: Vitex trifolia;Medicinal plant;Internode;Multiple shoot;Mass propagation;Verbenaceae
Issue Date: Jun-2011
Publisher: NISCAIR-CSIR, India
IPC Code: A61K 36/00
Abstract: A protocol was established for mass propagation of the valuable medicinal plant Vitex trifolia Linn.(Verbenaceae) through in vitro culture using leaf and internodal explants. The highest percentage (88%) of callus induction from internodal explants was observed on MS medium supplemented with 1.5mg/l 2, 4-D and 0.3mg/l KIN and highest percentage (95%) of shoot induction from callus was observed on MS medium supplemented with 2.0mg/l BAP and 0.3mg/l NAA. The in vitro raised shoots were successfully rooted in MS medium augmented with 0.5 mg/l IBA. The plantlets were subsequently hardened to green house conditions.
Page(s): 261-264
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJNPR Vol.2(2) [June 2011]

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