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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(3) [July 2011] >
| Title: | In vitro multiplication of Merton I. 793–An apple rootstock suitable for replantation |
| Authors: | Soni, Mohit
Thakur, Manisha
Modgil, Manju |
| Keywords: | Apple rootstocks
In vitro multiplication
Merton I 793
in vitro rooting
Micropropagation |
| Issue Date: | Jul-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In vitro shoot multiplication of Merton I. 793, a clonal apple rootstock,
was achieved through terminal/axillary bud culture using Murashige and Skoog
(MS) basal medium supplemented with 0.5-1.0 mg/L 6-benzyl amino purine (BA) and
0.1 mg/L indole-3-butyric acid (IBA). Time of collection of explants, duration
of surface sterilents and growth regulators added in the medium influenced the
survival of explants and their bud break. Although, the shoots were multiplied
on different concentrations of cytokinins and auxins, improved multiplication
was achieved with 0.5 mg/L BA and 0.01 mg/L IBA. Of the three types of explants
evaluated for shoot multiplication, shoot tips on elongation were found suitable
for rooting whereas, nodal cuttings and cluster of small shoots for further
multiplication. Low concentrations of α-naphthyl acetic acid (NAA) was proved
to be more effective for rooting as compared to IBA and indole-3-acetic acid
(IAA). To overcome profuse callus formation during rooting, activated charcoal
was added. In another experiment, auxin was discontinued from the rooting
medium after few days, thus, resulting in better rooting frequency (72%).
Thirty plantlets of Merton I. 793 were successfully hardened after 7 wks, with
around 80% survival rate. |
| Page(s): | 362-368 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(3) [July 2011]
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