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|Title:||Effect of organic solvents on solvent-tolerant Aeromonas hydrophila IBBPo8 and Pseudomonas aeruginosa IBBPo10|
|Authors:||Stancu, Mihaela Marilena|
|Abstract:||Alkanes (n-hexane, n-heptane) with logarithm of partition coefficient between n-octanol and water (log POW) 3.86 to 4.39 were less toxic to Aeromonas hydrophila IBBPo8 and Pseudomonas aeruginosa IBBPo10 as compared to aromatics (toluene, styrene, xylene isomers, ethylbenzene & propylbenzene) with log POW 2.64 to 3.69. The toxicity of 0.5% (v/v) second phase of organic solvents to these bacteria could be predictable on the basis of the solvents’ log POW. The tolerance, viability, adhesion and β-galactosidase activity of A. hydrophila IBBPo8 and P. aeruginosa IBBPo10 cells in the presence of 0.5% (v/v) organic solvents varied significantly. The results indicated that A. hydrophila IBBPo8 was more susceptible to organic solvents than P. aeruginosa IBBPo10, whereas both the bacterial strains harbour plasmids. A. hydrophila IBBPo8 did not posses hydrophobe/amphiphile efflux 1 (HAE1) transporter genes, while P. aeruginosa IBBPo10 did posses these genes. The adaptation mechanisms (modification of cell hydrophobicity, induction of β-galactosidase activity and changes in the membrane’s lipid and protein content) of bacterial cells, underlying solvent tolerance, in A. hydrophila IBBPo8 and P. aeruginosa IBBPo10 showed a complex response to the presence of 0.5% (v/v) organic solvents in the culture medium. Bacterial strains able to survive in the presence of organic solvents could be used in two-phase biotransformation systems with whole cells for adequate bioremediation of heavily contaminated sites and could be a source for new solvent-stable enzymes with different applications.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.10(3) [July 2011]|
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