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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(3) [July 2011] >
| Title: | Effect of organic solvents on solvent-tolerant Aeromonas hydrophila IBBPo8 and Pseudomonas aeruginosa IBBPo10 |
| Authors: | Stancu, Mihaela Marilena |
| Keywords: | Adaptation mechanisms
Bacteria
Organic solvents
Tolerance |
| Issue Date: | Jul-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Alkanes (n-hexane, n-heptane) with logarithm of partition
coefficient between n-octanol and water (log POW) 3.86
to 4.39 were less toxic to Aeromonas hydrophila IBBPo8 and Pseudomonas aeruginosa IBBPo10
as compared to aromatics (toluene, styrene, xylene isomers, ethylbenzene & propylbenzene)
with log POW 2.64
to 3.69. The toxicity of 0.5% (v/v) second phase of organic solvents to these
bacteria could be predictable on the basis of the solvents’ log POW.
The tolerance,
viability, adhesion and β-galactosidase
activity of A. hydrophila IBBPo8 and P. aeruginosa IBBPo10
cells in the presence of 0.5% (v/v) organic
solvents varied significantly. The results indicated that A. hydrophila IBBPo8 was more susceptible to
organic solvents than P. aeruginosa IBBPo10,
whereas both the bacterial strains harbour plasmids. A. hydrophila IBBPo8 did not posses hydrophobe/amphiphile efflux 1 (HAE1) transporter genes, while P. aeruginosa IBBPo10 did
posses these genes. The adaptation mechanisms (modification of cell
hydrophobicity, induction of β-galactosidase activity and changes in the
membrane’s lipid and protein content) of
bacterial cells, underlying solvent tolerance, in A. hydrophila IBBPo8 and
P. aeruginosa IBBPo10
showed a complex response to the
presence of 0.5% (v/v) organic solvents in the culture medium. Bacterial strains able to survive in the presence
of organic solvents could be used in two-phase biotransformation systems
with whole cells for adequate bioremediation
of heavily contaminated sites and could be a source for new solvent-stable enzymes with
different applications. |
| Page(s): | 352-361 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(3) [July 2011]
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