NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(3) [July 2011] >


Title: Effect of organic solvents on solvent-tolerant Aeromonas hydrophila IBBPo8 and Pseudomonas aeruginosa IBBPo10
Authors: Stancu, Mihaela Marilena
Keywords: Adaptation mechanisms
Bacteria
Organic solvents
Tolerance
Issue Date: Jul-2011
Publisher: NISCAIR-CSIR, India
Abstract: Alkanes (n-hexane, n-heptane) with logarithm of partition coefficient between n-octanol and water (log POW) 3.86 to 4.39 were less toxic to Aeromonas hydrophila IBBPo8 and Pseudomonas aeruginosa IBBPo10 as compared to aromatics (toluene, styrene, xylene isomers, ethylbenzene & propylbenzene) with log POW 2.64 to 3.69. The toxicity of 0.5% (v/v) second phase of organic solvents to these bacteria could be predictable on the basis of the solvents’ log POW. The tolerance, viability, adhesion and β-galactosidase activity of A. hydrophila IBBPo8 and P. aeruginosa IBBPo10 cells in the presence of 0.5% (v/v) organic solvents varied significantly. The results indicated that A. hydrophila IBBPo8 was more susceptible to organic solvents than P. aeruginosa IBBPo10, whereas both the bacterial strains harbour plasmids. A. hydrophila IBBPo8 did not posses hydrophobe/amphiphile efflux 1 (HAE1) transporter genes, while P. aeruginosa IBBPo10 did posses these genes. The adaptation mechanisms (modification of cell hydrophobicity, induction of β-galactosidase activity and changes in the membrane’s lipid and protein content) of bacterial cells, underlying solvent tolerance, in A. hydrophila IBBPo8 and P. aeruginosa IBBPo10 showed a complex response to the presence of 0.5% (v/v) organic solvents in the culture medium. Bacterial strains able to survive in the presence of organic solvents could be used in two-phase biotransformation systems with whole cells for adequate bioremediation of heavily contaminated sites and could be  a source for new solvent-stable enzymes with different applications.
Page(s): 352-361
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.10(3) [July 2011]

Files in This Item:

File Description SizeFormat
IJBT 10(3) 352-361.pdf286.76 kBAdobe PDFView/Open
 Current Page Visits: 853 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 620623 since 06-Feb-2009  Last updated on 13-Nov-2014Webmaster: nopr@niscair.res.in