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|Title:||Enhancement of dextransucrase activity of Pediococcus pentosaceus mutant SPAm1 by response surface methodology|
Response surface methodology
|Abstract:||In the present investigation, an optimal fermentation medium for the production of dextransucrase from mutant SPAm1 of a natural isolate of Pediococcus pentosaceus (GenBank Acc. No. EU569832) was developed following response surface method. A two-level Plackett-Burman design (PBD) and five-level Central composite design (CCD) was combined to optimize the medium composition for enhancement of the dextransucrase activity of SPAm1. A second-order model equation was suggested and validated experimentally. The model adequacy was very satisfactory as the coefficient of determination was 0.958. The optimum values for the tested variables were; sucrose 55.2 g/L; beef extract 2.3 g/L and Tween 80 8.3 mL/L. The predicted dextransucrase activity after response optimization was 15.9 U/mL, whereas the experimental value was 15.6 U/mL at 16 h of incubation, which showed a 3.2-fold enhancement over the enzyme activity (4.9 U/mL) of mutant SPAm1 given by the unoptimized medium. However, the increase in dextransucrase activity by SPAm1 after optimization of the medium was an outstanding 4.6-fold higher over that of the wild-type P. pentosaceus from the unoptimized medium (3.4 U/mL).|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.10(3) [July 2011]|
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