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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(3) [July 2011] >
| Title: | Extracellular polysaccharide production by a Rhizobium sp. isolated from legume herb Crotalaria saltiana Andr. |
| Authors: | Mukherjee, Sritama Ghosh, S Sadhu, S Ghosh, P Maiti, T K |
| Keywords: | Crotalaria saltiana Root nodule Rhizobium-legume Extracellular polysaccharides |
| Issue Date: | Jul-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A symbiont,
isolated from root nodules of Crotalaria saltiana Andr., was identified
as Rhizobium sp. It produced large amount of extracellular
polysaccharides (EPS) in broth culture in yeast extract mannitol (YEM) medium.
The EPS production and growth started simultaneously, though they have
different stationary phases. The symbiont produced maximum EPS (16 g/mL) when
the medium was supplemented with preferable carbon (glucose) 2%, nitrogen
(glycine) 0.1%, and vitamin (biotin) 1 g/mL. Attempts were made to optimize
the cultural requirements for growth and EPS production. The EPS produced by
the symbiont contained glucose and galactose. The possible role on Rhizobium
root nodule symboiosis has been discussed in the light of EPS production. |
| Page(s): | 340-345 |
| CC License: | CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(3) [July 2011]
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