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Title: Effect of culture conditions and kinetic studies on extracellular tannase production by <i style="">Lactobacillus plantarum</i> MTCC 1407
Authors: Kannan, Natarajan
Aravindan, Rajendran
Viruthagiri, Thangavelu
Keywords: Kinetics
Unstructured modeling
Issue Date: Jul-2011
Publisher: NISCAIR-CSIR, India
Abstract: Production of tannase and microbial biomass by <i style="">Lactobacillus plantarum</i> MTCC 1407 was studied in submerged batch fermentation. The tannase production was performed in different media compositions maintaining the fermentation conditions constant at 30°C, initial <i style="">p</i>H 5.5 and agitation speed at 120 rpm. Maximum tannase activity of 5.22 U mL<sup>-l</sup> was obtained at 24 h in M3 medium containing the following composition (g L<sup>-l</sup>): tannic acid, 10; glucose, 1; NH<sub>4</sub>Cl, 3; MgSO<sub>4</sub>.7H<sub>2</sub>O, 2; KH<sub>2</sub>PO<sub>4</sub>, 0.5; K<sub>2</sub>HPO<sub>4</sub>, 0.5; and CaCl<sub>2</sub>, l. The medium optimization studies show that the tannic acid (1% w/v) as inducer along with glucose (0.1% w/v) as carbon source gave maximum tannase activity. Unstructured kinetic models, namely, logistic model for cell growth and Luedeking-Piret model for tannase production, were used to predict the fermentation kinetics. The estimated values of the kinetic model parameters, α and β for tannase production indicated that the tannase production by <i style="">L. plantarum</i> was growth associated.
Description: 321-328
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.10(3) [July 2011]

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