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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(3) [July 2011] >
| Title: | Effect of culture conditions and kinetic studies on extracellular tannase production by Lactobacillus plantarum MTCC 1407 |
| Authors: | Kannan, Natarajan
Aravindan, Rajendran
Viruthagiri, Thangavelu |
| Keywords: | Kinetics
Optimization
Tannase
Unstructured modeling |
| Issue Date: | Jul-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Production of
tannase and microbial biomass by Lactobacillus
plantarum MTCC 1407 was studied in submerged batch fermentation. The
tannase production was performed in different media compositions maintaining
the fermentation conditions constant at 30°C, initial pH 5.5 and
agitation speed at 120 rpm. Maximum tannase activity of 5.22 U mL-l
was obtained at 24 h in M3 medium containing the following composition (g L-l):
tannic acid, 10; glucose, 1; NH4Cl,
3; MgSO4.7H2O, 2; KH2PO4, 0.5; K2HPO4,
0.5; and CaCl2, l. The medium optimization
studies show that the tannic acid
(1% w/v) as inducer along with glucose (0.1% w/v) as carbon source gave maximum
tannase activity. Unstructured kinetic models, namely, logistic model for
cell growth and Luedeking-Piret model for tannase production, were used to
predict the fermentation kinetics. The estimated values of the kinetic model
parameters, α and β for tannase production indicated that the tannase
production by L. plantarum was growth
associated. |
| Page(s): | 321-328 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(3) [July 2011]
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