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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(3) [July 2011] >
| Title: | Cloning of fibrinolytic protease-0 (Efp-0) gene from diverse earthworm individuals |
| Authors: | Sharma, Anshul
Sonah, Humira
Deshmukh, Rupesh K
Gupta, Navneet K
Singh, Nagendra K
Sharma, Tilak R |
| Keywords: | Earthworm
Eisenia fetida
Eudrilus eugeniae
Fibrinolytic protease
DNA sequencing
Structural characterization
Therapeutic agents |
| Issue Date: | Jul-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Fibrinolytic
enzyme has potential role as therapeutic agents for the treatment of some blood
clotting diseases. It is a novel oral-administered fibrinolytic agent used for
the prevention and treatment of cardiac and cerebrovascular diseases. As a drug, it has been widely studied
and used for clinical treatment. In the present study, we have cloned and sequenced partial Eisenia fetida fibrinolytic protease (Efp-0)
gene from three earthworm strains collected from diverse habitats. The gene was
amplified by using polymerase chain reaction and cloned in pGEM-T easy vector. The
size of Efp-0 gene, cloned
from the two strains of E. fetida and one strain of Eudrilus eugeniae,
was 658 bp, 658 bp and 660 bp,
respectively after sequencing,. Multiple sequence alignment of the cloned
gene along with gene sequences available in public database showed 21 SNPs and
5 InDels in the coding region. However, diversity observed in Efp-0 gene cloned
from different earthworm strains will provide basis for allele mining of this
gene. It is concluded that Efp-0 is a novel gene and can be utilized for industrial applications. The gene sequences obtained in this
study have been submitted in the GenBank (Accession no. GQ385193, GQ385194,
GQ385195). |
| Page(s): | 270-273 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(3) [July 2011]
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