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Title: Cloning of fibrinolytic protease-0 (Efp-0) gene from diverse earthworm individuals
Authors: Sharma, Anshul
Sonah, Humira
Deshmukh, Rupesh K
Gupta, Navneet K
Singh, Nagendra K
Sharma, Tilak R
Keywords: Earthworm;Eisenia fetida;Eudrilus eugeniae;Fibrinolytic protease;DNA sequencing;Structural characterization;Therapeutic agents
Issue Date: Jul-2011
Publisher: NISCAIR-CSIR, India
Abstract: Fibrinolytic enzyme has potential role as therapeutic agents for the treatment of some blood clotting diseases. It is a novel oral-administered fibrinolytic agent used for the prevention and treatment of cardiac and cerebrovascular diseases. As a drug, it has been widely studied and used for clinical treatment. In the present study, we have cloned and sequenced partial Eisenia fetida fibrinolytic protease (Efp-0) gene from three earthworm strains collected from diverse habitats. The gene was amplified by using polymerase chain reaction and cloned in pGEM-T easy vector. The size of Efp-0 gene, cloned from the two strains of E. fetida and one strain of Eudrilus eugeniae, was 658 bp, 658 bp and 660 bp, respectively after sequencing,. Multiple sequence alignment of the cloned gene along with gene sequences available in public database showed 21 SNPs and 5 InDels in the coding region. However, diversity observed in Efp-0 gene cloned from different earthworm strains will provide basis for allele mining of this gene. It is concluded that Efp-0 is a novel gene and can be utilized for industrial applications. The gene sequences obtained in this study have been submitted in the GenBank (Accession no. GQ385193, GQ385194, GQ385195).
Page(s): 270-273
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.10(3) [July 2011]

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