NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(3) [July 2011] >


Title: Cloning of fibrinolytic protease-0 (Efp-0) gene from diverse earthworm individuals
Authors: Sharma, Anshul
Sonah, Humira
Deshmukh, Rupesh K
Gupta, Navneet K
Singh, Nagendra K
Sharma, Tilak R
Keywords: Earthworm
Eisenia fetida
Eudrilus eugeniae
Fibrinolytic protease
DNA sequencing
Structural characterization
Therapeutic agents
Issue Date: Jul-2011
Publisher: NISCAIR-CSIR, India
Abstract: Fibrinolytic enzyme has potential role as therapeutic agents for the treatment of some blood clotting diseases. It is a novel oral-administered fibrinolytic agent used for the prevention and treatment of cardiac and cerebrovascular diseases. As a drug, it has been widely studied and used for clinical treatment. In the present study, we have cloned and sequenced partial Eisenia fetida fibrinolytic protease (Efp-0) gene from three earthworm strains collected from diverse habitats. The gene was amplified by using polymerase chain reaction and cloned in pGEM-T easy vector. The size of Efp-0 gene, cloned from the two strains of E. fetida and one strain of Eudrilus eugeniae, was 658 bp, 658 bp and 660 bp, respectively after sequencing,. Multiple sequence alignment of the cloned gene along with gene sequences available in public database showed 21 SNPs and 5 InDels in the coding region. However, diversity observed in Efp-0 gene cloned from different earthworm strains will provide basis for allele mining of this gene. It is concluded that Efp-0 is a novel gene and can be utilized for industrial applications. The gene sequences obtained in this study have been submitted in the GenBank (Accession no. GQ385193, GQ385194, GQ385195).
Page(s): 270-273
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.10(3) [July 2011]

Files in This Item:

File Description SizeFormat
IJBT 10(3) 270-273.pdf98.41 kBAdobe PDFView/Open
 Current Page Visits: 709 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 640210 since 06-Feb-2009  Last updated on 15-Dec-2014Webmaster: nopr@niscair.res.in