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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(3) [July 2011] >
| Title: | Cloning of chitinase gene from Bacillus thuringiensis |
| Authors: | Usharani, T R
Gowda, T K S |
| Keywords: | Bacillus thuringiensis
Colloidal chitin
Exochitinase
Fungitoxicity
Native PAGE |
| Issue Date: | Jul-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In the present investigation, 34 Bacillus
thuringiensis (Bt) isolates of
different agroclimatic condition were studied for fungitoxic characters and the
principle involved for fungitoxicity was identified in most of the cases as
exochitinases. The different Bt strains
were screened for fungitoxicity and chitin degrading activity employing 4
fungal pathogens. Biochemical
assay of the chitinase using glycol chitin as the substrate helped to identify
the enzyme as an exochitinase. The antifungal chitinase activity on
phyto-pathogenic Fusarium oxysporum f. sp. cubense was investigated in vitro by
fluorescent microscopic observation. PCR was employed using chitinase gene specific primers to
obtain the corresponding gene from Bt
isolates. The corresponding gene was cloned and sequenced for one of the
isolates and it was determined to be around 1129 bp, encoding a polypeptide of
360 amino acids. This gene was found to be highly homologous to that of
chitinase of B. cereus strain 28-9. |
| Page(s): | 264-269 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(3) [July 2011]
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