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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(3) [July 2011] >
| Title: | Molecular cloning, sequence analysis and tissue expression of penaeidin from white spot syndrome virus infected Penaeus monodon shrimps of Indian coastal region |
| Authors: | Rajesh, S
Anusha, S
Rajeshwari, R
Ramya, R
Kavitha, R
Narayanan, R B |
| Keywords: | Antimicrobial peptide
Hemocytes
Penaeidin
WSSV
Penaeus monodon |
| Issue Date: | Jul-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Immune related genes like penaeidins have
been characterized and the sequence diversity among penaeidins from various
shrimp isolates are well documented. However, no attempt has been made to study
the differential expression and sequence variations of these genes in Indian
context. Genes coding for penaeidin was PCR amplified and sequenced from
hemocyte cDNA of wild caught Penaeus
monodon shrimps from India
infected with white spot syndrome virus (WSSV). An elevated expression of
penaeidin was observed in the hemocytes of infected shrimps compared to
uninfected ones and tissue expression was confined only to the gills of
infected shrimps. The N-terminal signal peptide, which is a conserved feature
among all the penaeidins, was shown to have one substitution in the Indian
isolate. Besides this, there were ten substitutions following the signal
peptide region exclusive to the Indian isolate. Phylogenetic analysis suggested
a close relatedness of penaeidin from the Indian isolate with the homologs from
other P. monodon and P. chinenis and a distant origin with
the other isolates. An elevated level of penaeidin during WSSV infection
suggests that penaeidin might have a function in defending shrimps against
viral infections. |
| Page(s): | 257-263 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(3) [July 2011]
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