NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(3) [July 2011] >

Title: Molecular cloning, sequence analysis and tissue expression of penaeidin from white spot syndrome virus infected Penaeus monodon shrimps of Indian coastal region
Authors: Rajesh, S
Anusha, S
Rajeshwari, R
Ramya, R
Kavitha, R
Narayanan, R B
Keywords: Antimicrobial peptide
Penaeus monodon
Issue Date: Jul-2011
Publisher: NISCAIR-CSIR, India
Abstract: Immune related genes like penaeidins have been characterized and the sequence diversity among penaeidins from various shrimp isolates are well documented. However, no attempt has been made to study the differential expression and sequence variations of these genes in Indian context. Genes coding for penaeidin was PCR amplified and sequenced from hemocyte cDNA of wild caught Penaeus monodon shrimps from India infected with white spot syndrome virus (WSSV). An elevated expression of penaeidin was observed in the hemocytes of infected shrimps compared to uninfected ones and tissue expression was confined only to the gills of infected shrimps. The N-terminal signal peptide, which is a conserved feature among all the penaeidins, was shown to have one substitution in the Indian isolate. Besides this, there were ten substitutions following the signal peptide region exclusive to the Indian isolate. Phylogenetic analysis suggested a close relatedness of penaeidin from the Indian isolate with the homologs from other P. monodon and P. chinenis and a distant origin with the other isolates. An elevated level of penaeidin during WSSV infection suggests that penaeidin might have a function in defending shrimps against viral infections.
Page(s): 257-263
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.10(3) [July 2011]

Files in This Item:

File Description SizeFormat
IJBT 10(3) 257-263.pdf951.78 kBAdobe PDFView/Open
 Current Page Visits: 128 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 169546 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: