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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(3) [July 2011] >
| Title: | Plasmid borne gene of Bacillus pumillus MTCC 7615 responsible for fungal antagonism towards Rhizoctonia solani |
| Authors: | Padaria, Jasdeep Chatrath Kapoor, Vikram |
| Keywords: | Bacillus pumillus MTCC7615 Rhizoctonia solani Antagonism Tn5 mutant IPCR |
| Issue Date: | Jul-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A bacterial
isolate, Bacillus pumillus MTCC7615,
obtained from rice field was characterized as antagonistic
against the fungal pathogen, Rhizoctonia
solani, under in vitro conditions
in previous studies in the lab. The present study aims at using the IPCR
approach for identification of B. pumillus MTCC7615 gene(s) responsible
for
antagonism towards the fungal pathogen, R.
solani, by analysis of its mutant B. pumillus A1a. B. pumillus MTCC7615
may be harbouring more than one biocontrol gene on genomic and/or plasmid DNA.
The sequence data of the
identified plasmid-borne gene (GenBank, NCBI accession GU126748.1) showed
extensive homology with conserved hypothetical protein of Selenomonas flueggei and other related bacteria. There is a need
for understanding the
molecular mechanism of the biological control approach (BCA) to develop
transgenics that are resistant to various
biotic stresses. |
| Page(s): | 316-320 |
| CC License: | CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(3) [July 2011]
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