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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.10 [2011] >
IJBT Vol.10(3) [July 2011] >
| Title: | Detection of natural genetic diversity of Gaultheria fragrantissima landraces by RAPDs: An endangered woody oil bearing plant of Indo-China Himalayas |
| Authors: | Bantawa, Pranay
Das, Akan
Ghosh, Partha Deb
Mondal, Tapan Kumar |
| Keywords: | Aromatic plant
Genetic diversity
Gaultheria frarentissima
Germplasm characterization
RAPD |
| Issue Date: | Jul-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Indian winter green (Gaultheria
fragrantissima) (Erieaceae) is an endangered aromatic plant containing
highest amount of methyl salicylate among the plant kingdom. Earlier, wide
ranging oil bearing capabilities of the populations of
G. fragrantissima were identified by
us. Therefore, random amplified
polymorphic DNA (RAPD) was used to fingerprint 23 genotypes of eastern
Himalayas. A high degree of polymorphism was detected with 5 informative
primers namely, OPE-03, OPE-04, OPE-7, OPE-18 and OPE-20. Among these, OPE-3
produced the highest (87.5%) while OPE-07 produced the lowest (53.85%) degree
of polymorphism. While the extent of
total variability was recorded to be 68.52%, the inter- and intra-population
variability was found to be 53% and 46%, respectively. A resemblance matrix was developed, using Simple
Matching Coefficient, to compute cluster analysis. It was
concluded that the RAPD markers detected a sufficient degree of polymorphism to
differentiate among the Gaultheria genotypes, which will be useful for
conservation of this endangered plant species. |
| Page(s): | 294-300 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.10(3) [July 2011]
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