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Title: Analytical Methods
Issue Date: Apr-2011
Publisher: NISCAIR-CSIR, India
Abstract: High-performance liquid chromatographic method with diode-array detection (HPLC–DAD) was optimised and validated for determination of tetracyclines (TCs) residue in chicken meat and liver through evaluating each step of various methods. The principle steps involved ultrasonic-assisted extractionof TCs from chicken samples by 2 ml of 20% trichloroacetic acid and citrate buffer (pH 4) which gave a clearer supernatant and high recovery, followed by centrifugation and purification on SPE (Strata C18-E cartridge) using 10 ml of 0.01 M methanolic oxalic acid for TCs elution.
Page(s): 118-119
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
Source:NPARR Vol.2(2) [April 2011]

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