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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(07) [July 2011] >
| Title: | A novel method of plasmid isolation using laundry detergent |
| Authors: | Yadav, P
Yadav, A
Garg, V
Datta, T K
Goswami, S L
De, S |
| Keywords: | Plasmid
Laundry detergent
Vector |
| Issue Date: | Jul-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Since the
discovery of plasmid, various methods have been developed to isolate plasmid
DNA. All the methods have one common and important target of isolating plasmid
DNA of high quality and quantity in less time. These methods are not
completely safe because of use of toxic chemicals
compounds. The developed protocol for plasmid extraction is based on the
alkaline lysis method of plasmid preparation (extraction at pH
8.0) with slight modifications.
Cell lysis reagent sodium dodecyl
sulfate is replaced by lipase enzyme present in laundry detergent.
A good plasmid preparation can be made, which is well suited for subsequent
molecular biology applications. By taking safety measures on count,
contaminants like, RNA and protein can be completely avoided with maximized
plasmid yield. The resultant plasmid quality
and quantity can be well comparable to other prevalent methods. |
| Page(s): | 558-560 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(07) [July 2011]
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