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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(07) [July 2011] >
| Title: | Inhibition of Naja nigricolis (Reinhardt) venom protease activity by Luffa egyptiaca (Mill) and Nicotiana rustica (Linn) extracts |
| Authors: | Ibrahim, M A
Aliyu, A B
Abusufiyanu, A
Bashir, M
Sallau, A B |
| Keywords: | Luffa egyptiaca
Naja nigricolis
Nicotiana rustica
Protease
Venom |
| Issue Date: | Jul-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Luffa
egyptiaca and Nicotiana
rustica are used in traditional medicine to treat snakebites and were evaluated
for inhibitory activities on Naja
nigricolis venom protease. The aqueous and ethanolic extracts of L. egyptiaca significantly reduced the
maximum velocity (Vmax) and the computed index of physiological
efficiency (Kcat) of the
enzyme in a dose dependent fashion. The protease activity was non-competitively
inhibited by the aqueous extract of N.
rustica with the Vmax significantly decreased and the KM
remained unchanged. However, the N.
rustica ethanol extract completely inhibited the protease activity. Ethyl
acetate fractions partitioned from ethanol extracts of both plants were also found
to completely inhibit the N. nigricolis venom
protease activity at 0.1 and 0.05%.
The use of these plants could be important in the treatment of snakebites. |
| Page(s): | 552-554 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(07) [July 2011]
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