Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/11992
Title: Gas-chromatography and electroantennogram analysis of saturated hydrocarbons of cruciferous host plants and host larval body extracts of <i style="">Plutella xylostella</i> for behavioural manipulation of <i style="">Cotesia plutellae </i>
Authors: Seenivasagan, T
Paul, A V Navarajan
Keywords: Behaviour manipulation
<i style="">Cotesia plutellae</i>
Electroantennogram
Flight orientation and landing
Gas chromatography
Hydrocarbons
<i style="">Plutella xylostella</i>
Wind tunnel
Issue Date: May-2011
Publisher: NISCAIR-CSIR, India
Abstract: Saturated hydrocarbons (SHC) of five cruciferous host plants viz., cabbage, cauliflower, broccoli, knol khol and Brussels sprout and the larvae of diamondback moth (DBM),<i style=""> Plutella xylostella</i> reared on these host plants were identified through gas-chromatography. The hydrocarbon profile of host plants and larval body extract of DBM reared on respective host plants revealed a wide variation in quantity as well as quality. Long chain hydrocarbons C<sub>26</sub>-C<sub>30</sub> were detected in all the extracts. In electroantennogram (EAG) studies, SHCs at 10<sup>-3</sup>g dose elicited differential EAG response in the antennal receptors of gravid <i style="">Cotesia plutellae</i> females. Tricosane (C<sub>23</sub>) and hexacosane (C<sub>26</sub>) elicited 10-fold increased EAG response compared to control stimulus. Long chain hydrocarbons C<sub>27</sub>, C<sub>28</sub> and C<sub>29</sub> elicited, 6-7 fold increased responses. The sensitivity of antenna was 4-5 folds for C<sub>25</sub>, C<sub>14</sub>, C<sub>24</sub>, C<sub>15</sub> and C<sub>30</sub>, while the short chain hydrocarbons elicited 2-3 fold increased EAG responses. Dual choice flight orientation experiments in a wind tunnel revealed that the gravid <i style="">C. plutellae</i> females preferred the odour of C<sub>16</sub>, C<sub>26</sub>, C<sub>29</sub>, C<sub>15, </sub>C<sub>21</sub>, C<sub>23</sub>, C<sub>30</sub>, C<sub>27</sub>, C<sub>24</sub> and C<sub>22</sub> as 60-70% females oriented and landed on SHC treated substrate compared to control odour, while the odour of eicosane (C<sub>20</sub>), pentacosane (C<sub>25</sub>) and octacosane (C<sub>28</sub>) were not preferred by the females.
Description: 375-386
URI: http://hdl.handle.net/123456789/11992
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.49(05) [May 2011]

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