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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.48 [2011] >
IJBB Vol.48(3) [June 2011] >
| Title: | Lindane-induced biochemical perturbations in rat serum and attenuation by omega-3 and Nigella sativa seed oil |
| Authors: | Attia, Ahmed M
El-Banna, Sabah G
Nomeir, Farid R
El-Basser, Mohammed I Abd |
| Keywords: | Antioxidant enzymes
Lindane
Lipid profile
Nigella sativa oil
Omega-3
Oxidative stress
Hepato-specific enzymes |
| Issue Date: | Jun-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Lindane ( -hexachlorocyclohexane,
-HCH), a highly persistent
organochlorine insecticide is neurotoxic at acute doses and has been reported
to induce oxidative stress in cells and tissues. In this study, we investigated
the antioxidant property of Nigella sativa seed oil (N.O) and omega-3
polyunsaturated fatty acids ( 3) against -HCH-induced
oxidative hepatic and renal damage in male rats serum. Rats were orally given
sublethal dose of -HCH (12
mg/kg, 24 h prior to decapitation), while N.O (0.3 ml/kg) and 3
(20 mg/kg) were given every 48 h for 20 days single or together, or also
combined with
-HCH. -HCH
caused a significant increase in the levels of serum total lipids, cholesterol, and triglycerides by 49, 61 and 30% respectively,
while HDL-cholesterol decreased by 45% compared to control group. Pretreatment
with 3
and N.O prior -HCH
administration re-established the altered biochemical features and alleviated
the harmful effects of g-HCH on lipid
profile. The concentration of serum total protein and albumin was significantly
decreased by 35 and 45% respectively in rats treated with -HCH compared to control. -HCH also
caused hepatic and renal damage, as observed from the elevated serum levels of
urea, creatinine, total bilirubin and
uric acid contents and aminotransferases (AST and ALT), phosphatases
(ACP and ALP) and lactate dehydrogenase (LDH) activities.
Co-administration of 3 and N.O reversed the hazardous effects
induced by -HCH on the liver
and kidney and also protected acetylcholinesterase from the inhibitory action
of -HCH as well as suppressed the
lipid peroxidation. Thus, the results show that 3 and N.O might
prevent oxidative stress and attenuate the changes in the biochemical
parameters induced by -HCH in male rats. |
| Page(s): | 184-190 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.48(3) [June 2011]
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