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Title: Lindane-induced biochemical perturbations in rat serum and attenuation by omega-3 and <i>Nigella sativa</i> seed oil
Authors: Attia, Ahmed M
El-Banna, Sabah G
Nomeir, Farid R
El-Basser, Mohammed I Abd
Keywords: Antioxidant enzymes
Lipid profile
<i>Nigella sativa</i> oil
Oxidative stress
Hepato-specific enzymes
Issue Date: Jun-2011
Publisher: NISCAIR-CSIR, India
Abstract: Lindane (<img src='/image/spc_char/gamma.gif' border=0>-hexachlorocyclohexane, <img src='/image/spc_char/gamma.gif' border=0>-HCH), a highly persistent organochlorine insecticide is neurotoxic at acute doses and has been reported to induce oxidative stress in cells and tissues. In this study, we investigated the antioxidant property of Nigella sativa seed oil (N.O) and<b> </b>omega-3 polyunsaturated fatty acids (<img src='' border=0><sub>3</sub>) against <img src='/image/spc_char/gamma.gif' border=0>-HCH-induced oxidative hepatic and renal damage in male rats serum. Rats were orally given sublethal dose of <img src='/image/spc_char/gamma.gif' border=0>-HCH (12 mg/kg, 24 h prior to decapitation), while N.O (0.3 ml/kg) and <img src='' border=0><sub>3</sub> (20 mg/kg) were given every 48 h for 20 days single or together, or also combined with <img src='/image/spc_char/gamma.gif' border=0>-HCH. <img src='/image/spc_char/gamma.gif' border=0>-HCH caused a significant increase in the levels of serum total lipids, cholesterol, and triglycerides by 49, 61 and 30% respectively, while HDL-cholesterol decreased by 45% compared to control group. Pretreatment with <img src='' border=0><sub>3</sub> and N.O prior <img src='/image/spc_char/gamma.gif' border=0>-HCH administration re-established the altered biochemical features and alleviated the harmful effects of g-HCH on lipid profile. The concentration of serum total protein and albumin was significantly decreased by 35 and 45% respectively in rats treated with <img src='/image/spc_char/gamma.gif' border=0>-HCH compared to control. <img src='/image/spc_char/gamma.gif' border=0>-HCH also caused hepatic and renal damage, as observed from the elevated serum levels of urea, creatinine, total bilirubin and uric acid contents and aminotransferases (AST and ALT), phosphatases (ACP and ALP) and lactate dehydrogenase (LDH) activities. Co-administration of <img src='' border=0><sub>3</sub> and N.O reversed the hazardous effects induced by <img src='/image/spc_char/gamma.gif' border=0>-HCH on the liver and kidney and also protected acetylcholinesterase from the inhibitory action of <img src='/image/spc_char/gamma.gif' border=0>-HCH as well as suppressed the lipid peroxidation. Thus, the results show that <img src='' border=0><sub>3</sub> and N.O might prevent oxidative stress and attenuate the changes in the biochemical parameters induced by <img src='/image/spc_char/gamma.gif' border=0>-HCH in male rats.
Description: 184-190
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.48(3) [June 2011]

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