19-Jun-2013
22:51:42 IST
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NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.48 [2011] >
IJBB Vol.48(3) [June 2011] >
| Title: | Anti-venom potential of aqueous extract of stem bark of Mangifera indica L. against Daboia russellii (Russell’s viper) venom |
| Authors: | Dhananjaya, B L
Zameer, F
Girish, K S
D`Souza, Cletus J M |
| Keywords: | Mangifera indica
Daboia russellii
Snake venom neutralization
Cocktail of inhibitors
Antivenom potential |
| Issue Date: | Jun-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Several plant extracts rich in
pharmacologically active compounds have shown to antagonize venom of several
species. Mangifera indica has been used against snakebite by the
traditional healers, However, there is paucity of scientific data in support.
In this study, we evaluated the antivenom potential of aqueous extract of stem
bark of M. indica against D. russellii venom-induced
pharmacological effects such as life myotoxicity, edema, LD50 etc.
The extract inhibited
the phospholipase, protease, hyaluronidase, 5`nucleotidase, ATPase and alkaline
phosphomonoesterase activities with varying IC50 values.
It significantly inhibited both metalloproteases and serine proteases
activities. Further, the extract significantly reduced the myotoxicity of the venom,
as evident by the reduction of serum creatin kinase and lactate
dehydrogenase
activities. Though the extract completely inhibited in vitro PLA2
activity, it was unable to completely inhibit in situ hemolytic and in
vivo edema-inducing activities, usually brought about by PLA2s.
In lethality studies, co-injection of the venom preincubated with the extract
showed higher protection than the independent injection of venom, followed by
the extract in the mice. However, in both the cases the extract -a cocktail of
inhibitors significantly increased the survival time, when compared to that of
mice injected (i.p) with the venom alone. These results encourage further
studies on the potential use of cocktail of inhibitors in improving the
treatment of snake envenomation. Further, this study
substantiates the use of M. indica as an antidote against snakebite by
the traditional healers. |
| Page(s): | 175-183 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.48(3) [June 2011]
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