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IJBB Vol.44(4) [August 2007] >


Title: Metabolism of arachidonic acid in sheep uterus: In vitro studies
Authors: Padma, A Sai
Agarwal, S
Reddy, D Bharat
Prasad, T Sandeep
Reddanna, P
Keywords: Arachidonic acid metabosism
Lipoxygenase
Cyclooxygenase
Prostaglandins
HPETEs and HETEs
Issue Date: Aug-2007
Publisher: CSIR
Abstract: Arachidonic acid (AA) metabolism in the non-pregnant sheep uterus was studied in vitro using conventional chromatographic and HPLC techniques. High expression of both lipoxygenase (LOX) as well as cyclooxygenase (COX) enzymes and their activities was found in the uterine tissues. On incubation of uterine enymes with AA, the LOX products formed were identified as 5-hydroperoxyeicosatetraenoic acid (5-HPETE), 12- and 15-hydroxyeicosatetraenoic acids (12- and 15-HETEs), based on their separation on TLC and HPLC. By employing differential salt precipitation techniques, the LOXs generating products 5-HPETE (5-LOX), 12-HETE and 15-HETE (12- and 15-dual LOX) were isolated. Based on their analysis on TLC, the COX products formed were identified as prostaglandins - PGF2α and prostacyclin derivative 6-keto PGF1α. The study forms the first report on the comprehensive analysis on the metabolism of AA in sheep uterus in vitro via the LOX and COX pathways.
Page(s): 216-222
ISSN: 0301-1208
Source:IJBB Vol.44(4) [August 2007]

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