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Title: Differential gene expression in pepper (Capsicum annuum) exposed to UV-B
Authors: Lai, Yan
Xu, Bo
He, Li
Lin, Ming
Cao, Lei
Mou, Shaoliang
Wu, Yang
He, Shuilin
Keywords: Capcab;Capsicum annuum;cDNA-AFLP;TDF;UV-B
Issue Date: Jun-2011
Publisher: NISCAIR-CSIR, India
Abstract: In the present paper, complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) was used to examine and identify differentially expressed genes in Capsicum annuum exposed to UV-B irradiation. Around 4000 transcript derived fragments (TDFs) were visualized and in total 183 TDFs were isolated, sequenced and analyzed by Blast 2 go. Among these TDFs, 84 of them showed homology to known genes. There were 43 TDFs showing up-regulated expression, 24 TDFs showing down-regulated expression and 27 TDFs showing both up-regulated and down-regulated expression, respectively. Some of these TDFs were found to be in response/related to UV-B stress, including carbonic anhydrase, calcium-dependent protein, thionin-like protein, bzip protein and so on. In particular, chlorophyll a/b binding protein (Capcab) responding to UV-B stress was cloned. It was concluded that Capcab could play a protective role in plant anti-UV-B and maintaining photosynthetic rate under UV-B stress.
Page(s): 429-437
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.49(06) [June 2011]

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