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http://nopr.niscair.res.in/handle/123456789/11741
Title: | Differential gene expression in pepper (Capsicum annuum) exposed to UV-B |
Authors: | Lai, Yan Xu, Bo He, Li Lin, Ming Cao, Lei Mou, Shaoliang Wu, Yang He, Shuilin |
Keywords: | Capcab;Capsicum annuum;cDNA-AFLP;TDF;UV-B |
Issue Date: | Jun-2011 |
Publisher: | NISCAIR-CSIR, India |
Abstract: | In the present paper, complementary DNA-amplified fragment length polymorphism (cDNA-AFLP) was used to examine and identify differentially expressed genes in Capsicum annuum exposed to UV-B irradiation. Around 4000 transcript derived fragments (TDFs) were visualized and in total 183 TDFs were isolated, sequenced and analyzed by Blast 2 go. Among these TDFs, 84 of them showed homology to known genes. There were 43 TDFs showing up-regulated expression, 24 TDFs showing down-regulated expression and 27 TDFs showing both up-regulated and down-regulated expression, respectively. Some of these TDFs were found to be in response/related to UV-B stress, including carbonic anhydrase, calcium-dependent protein, thionin-like protein, bzip protein and so on. In particular, chlorophyll a/b binding protein (Capcab) responding to UV-B stress was cloned. It was concluded that Capcab could play a protective role in plant anti-UV-B and maintaining photosynthetic rate under UV-B stress. |
Page(s): | 429-437 |
URI: | http://hdl.handle.net/123456789/11741 |
ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
Appears in Collections: | IJEB Vol.49(06) [June 2011] |
Files in This Item:
File | Description | Size | Format | |
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IJEB 49(6) 429-437.pdf | 505.4 kB | Adobe PDF | View/Open |
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