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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(06) [June 2011] >
| Title: | Differential gene expression in pepper (Capsicum annuum) exposed to UV-B |
| Authors: | Lai, Yan
Xu, Bo
He, Li
Lin, Ming
Cao, Lei
Mou, Shaoliang
Wu, Yang
He, Shuilin |
| Keywords: | Capcab
Capsicum annuum
cDNA-AFLP
TDF
UV-B |
| Issue Date: | Jun-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | In the present paper, complementary
DNA-amplified fragment length polymorphism (cDNA-AFLP) was used to examine and
identify differentially expressed genes in Capsicum
annuum exposed to UV-B irradiation. Around 4000 transcript derived
fragments (TDFs) were visualized and in total 183 TDFs were isolated, sequenced
and analyzed by Blast 2 go. Among these TDFs, 84 of them showed homology to
known genes. There were 43 TDFs showing up-regulated
expression, 24 TDFs showing down-regulated expression and 27 TDFs showing
both up-regulated and down-regulated expression, respectively. Some of these
TDFs were found to be in response/related to UV-B stress, including carbonic
anhydrase, calcium-dependent protein, thionin-like protein, bzip protein and so
on. In particular, chlorophyll a/b binding protein (Capcab) responding to UV-B stress was cloned. It was concluded that
Capcab could play a protective role
in plant anti-UV-B and maintaining photosynthetic rate under UV-B stress. |
| Page(s): | 429-437 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(06) [June 2011]
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