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Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(06) [June 2011] >
| Title: | No effect of low-level lasers on in vitro myoblast culture |
| Authors: | Mesquita-Ferrari, Raquel A
Ribeiro, Rafael
Souza, Nadhia H C
Silva, Camila A A
Martins, Manoela D
Bussadori, Sandra K
Fernandes, Kristianne P S |
| Keywords: | C2C12 cells
Cell proliferation
Low-level lasers
Myoblasts |
| Issue Date: | Jun-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Effects of phototherapy
using low-level lasers depend on irradiation parameters and the type of laser
used. The aim of the present study was to evaluate the effect of phototherapy
on the proliferation of cultured C2C12 myoblasts under different nutritional
conditions using low-level GaAlAs and InGaAlP lasers with different parameters
and incubation periods. C2C12 cells cultured in regular and nutrient-deficient
medium were irradiated with low-level GaAlAs (780 nm) and InGaAlP (660 nm)
lasers with energy densities of 3.8, 6.3 and 10 J/cm2, and 3.8, 10
and 17.5 J/cm2, respectively. Cell proliferation was assessed 48 and
72 h after irradiation by MTT assay. There were no significant differences in
cell proliferation between laser-treated myoblasts and control cultures for any
of the parameters and incubation periods. Further studies are necessary to determine the correct laser
parameters for optimizing the biostimulation of myoblasts. |
| Page(s): | 423-428 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(06) [June 2011]
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