NISCAIR Online Periodicals Repository

Research Journals >
Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.70 [2011] >
JSIR Vol.70(05) [May 2011] >

Title: Immobilization of peroxidase on a cost free support from salt fractionated proteins of bitter gourd
Authors: Karim, Zoheb
Husain, Qayyum
Keywords: Bitter gourd peroxidase
Cost free support
Fly ash
Issue Date: May-2011
Publisher: NISCAIR-CSIR, India
Abstract: This study presents direct immobilization of peroxidases from ammonium sulphate fractionated proteins of bitter gourd on cost free support, fly ash. Activated fly ash (, 0.84) was quite effective in high yield immobilization of peroxidase from bitter gourd and it bind nearly 1113 enzyme units per g of support in sodium acetate buffer, pH 5.0. This enzyme bound very strongly on this support and it did not detach even in the presence of 1.0M NaCl. Fly ash adsorbed bitter gourd peroxidase retained 50% activity after treatment with 2.5 mg trypsin/ml of incubation mixture for 1 h at 40°C while soluble enzyme lost nearly 65% of initial activity under similar incubation conditions. FT-IR spectra of fly ash and adsorbed peroxidase showed different peaks at respective wavenumbers.
Page(s): 352-359
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-1084 (Online); 0022-4456 (Print)
Source:JSIR Vol.70(05) [May 2011]

Files in This Item:

File Description SizeFormat
JSIR 70(5) 352-359.pdf131.32 kBAdobe PDFView/Open
 Current Page Visits: 1033 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 171340 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: