|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(05) [May 2011] >
| Title: | Antimicrobial activity of protease inhibitor from leaves of Coccinia grandis (L.) Voigt. |
| Authors: | Satheesh, L Shilpa
Murugan, K |
| Keywords: | Antimicrobial activity
Chromatography
Coccinia grandis
Cytotoxicity
Protease inhibitors
Purification |
| Issue Date: | May-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Antimicrobial activity of protease inhibitor isolated
from Coccinia grandis (L.) Voigt. has been reported. A 14.3 kDa protease inhibitor (PI) was isolated and purified to
homogeneity by ammonium sulfate precipitation (20-85% saturation), sephadex
G-75, DEAE sepharose column and trypsin-sepharose affinity chromatography from
the leaves of C. grandis. The purity
was checked by reverse phase high performance liquid chromatography. PI
exhibited marked growth inhibitory effects on colon cell lines in a
dose-dependent manner. PI was thermostable and showed antimicrobial activity
without hemolytic activity. PI strongly inhibited pathogenic microbial strains,
including Staphylococcus aureus, Klebsiella pneumoniae, Proteus vulgaris,
Eschershia coli, Bacillus subtilis and pathogenic fungus Candida albicans, Mucor
indicus, Penicillium notatum, Aspergillus flavus and Cryptococcus neoformans. Examination by
bright field microscopy showed inhibition of mycelial growth and sporulation.
Morphologically, PI treated fungus showed a significant shrinkage of hyphal
tips. Reduced PI completely lost its activity indicating that disulfide bridge
is essential for its protease inhibitory and antifungal activity. Results
reported in this study suggested that PI may be an excellent candidate for
development of novel oral or other anti-infective agents. |
| Page(s): | 366-374 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(05) [May 2011]
|
|
