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Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(05) [May 2011] >
| Title: | Telomere instability caused by subtelomeric Y′ amplification and rearrangements in Saccharomyces cerevisiae (ku70 tel1 and ku70 rad50) double mutants |
| Authors: | Ruiz-Gómez, Miguel J |
| Keywords: | Chromosome stability
Telomere
Y′ amplification
Yeast |
| Issue Date: | May-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | Telomeres solve the end-replication problem. Previous results suggested a
relation between Yku70/80 and proteins Tel1 and Rad50 in telomere stabilization.
Inactivation of any of these genes lead to a shortening of telomeres, while in ku70 tel1 or ku70 rad50 double mutants a drastic amplification of Y' elements
was found. The biological significance of this observation is not clear. To
further characterize Y′ amplification 25 strains and isolates of S. cerevisiae were analyzed. As
expected, amplification was seen in yku70
tel1 and yku70 rad50 double
mutants, but not in other strains. The extent of Y′ amplification was also
tested to determine if excessive numbers of Y′ repeats appear. A variation in
chromosome lengths within the population of cells has been found. Hybridisation
study indicated that chromosomes only increase in length in these double
mutants, but never get shorter. A high degree of variability was observed in
single cell clones, in spite of their close relationship, indicating that
alterations in subtelomeric regions are not stable but occur continuously in
these mutants. Therefore, these genes are essential to chromosome stability |
| Page(s): | 324-331 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(05) [May 2011]
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