|
NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR) >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Experimental Biology (IJEB) >
IJEB Vol.49 [2011] >
IJEB Vol.49(05) [May 2011] >
| Title: | Effect of 8-alkylberberine homologues on erythrocyte membrane |
| Authors: | Yong, Yang
Xiao-li, Ye
Bao-shun, Zhang
Xue-gang, Li |
| Keywords: | 8-alkylberberine homologues (Ber-C8-n)
Cell membrane
Fluorescence of membrane protein
Membrane fluidity |
| Issue Date: | May-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | 8-alkylberberine
homologues (Ber-C8-n,
where n indicates carbon atom number
of gaseous normal alkyl at 8 position, n =0, 2, 4, 6, 8, 10, 12, or 16) were synthesized and their effects on the hemolysis of rabbit erythrocyte, the fluidity of membrane and the fluorescence of membrane
protein were investigated by fluorescence analysis technique. Ber-C8-n
with mediate length alkyl (4<n<10) exhibited obvious
hemolysis effect on
rabbit erythrocyte
when their concentration exceed 1.25×10-4 mol/L, and Ber-C8-8 displayed the highest hemolysis effect among all tested homologues. All of Ber-C8-n influenced the fluidity of erythrocyte membrane to different extents, which exhibited an obvious dose-effect relationship. The effect of
Ber-C8-n on fluidity increased as the length of alkyl chain was elongated and decreased gradually
when the alkyl carbon atoms exceeded 8. The fluorescence of erythrocyte membrane protein was quenched by Ber-C8-n,
which showed a similar changing tendency on membrane fluidity. Experiments in vitro suggested that disturbing effects of Ber-C8-n on the
conformation and function of membrane protein leaded to the changes of membrane
fluidity and stability, and then the membrane was broken down. |
| Page(s): | 319-323 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Source: | IJEB Vol.49(05) [May 2011]
|
|
