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Title: Effect of 8-alkylberberine homologues on erythrocyte membrane
Authors: Yong, Yang
Xiao-li, Ye
Bao-shun, Zhang
Xue-gang, Li
Keywords: 8-alkylberberine homologues (Ber-C8-n);Cell membrane;Fluorescence of membrane protein;Membrane fluidity
Issue Date: May-2011
Publisher: NISCAIR-CSIR, India
Abstract: 8-alkylberberine homologues (Ber-C8-n, where n indicates carbon atom number of gaseous normal alkyl at 8 position, n =0, 2, 4, 6, 8, 10, 12, or 16) were synthesized and their effects on the hemolysis of rabbit erythrocyte, the fluidity of membrane and the fluorescence of membrane protein were investigated by fluorescence analysis technique. Ber-C8-n with mediate length alkyl (4<n<10) exhibited obvious hemolysis effect on rabbit erythrocyte when their concentration exceed 1.25×10-4 mol/L, and Ber-C8-8 displayed the highest hemolysis effect among all tested homologues. All of Ber-C8-n influenced the fluidity of erythrocyte membrane to different extents, which exhibited an obvious dose-effect relationship. The effect of Ber-C8-n on fluidity increased as the length of alkyl chain was elongated and decreased gradually when the alkyl carbon atoms exceeded 8. The fluorescence of erythrocyte membrane protein was quenched by Ber-C8-n, which showed a similar changing tendency on membrane fluidity. Experiments in vitro suggested that disturbing effects of Ber-C8-n on the conformation and function of membrane protein leaded to the changes of membrane fluidity and stability, and then the membrane was broken down.
Page(s): 319-323
ISSN: 0975-1009 (Online); 0019-5189 (Print)
Appears in Collections:IJEB Vol.49(05) [May 2011]

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