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|Title:||Effect of 8-alkylberberine homologues on erythrocyte membrane|
|Keywords:||8-alkylberberine homologues (Ber-C8-<i>n</i>)|
Fluorescence of membrane protein
|Abstract:||8-alkylberberine homologues (Ber-C8-<i>n</i>, where <i style="">n</i> indicates carbon atom number of gaseous normal alkyl at 8 position, <i>n </i>=0, 2, 4, 6, 8, 10, 12, or 16) were synthesized and their effects on the hemolysis of rabbit erythrocyte, the fluidity of membrane and the fluorescence of membrane protein were investigated by fluorescence analysis technique. Ber-C8-<i>n</i> with mediate length alkyl (4<<i style="">n</i><10) exhibited obvious hemolysis effect on rabbit erythrocyte when their concentration exceed 1.25×10<sup>-4</sup> mol/L, and Ber-C8-8 displayed the highest hemolysis effect among all tested homologues. All of Ber-C8-<i>n</i> influenced the fluidity of erythrocyte membrane to different extents, which exhibited an obvious dose-effect relationship. The effect of Ber-C8-<i>n</i> on fluidity increased as the length of alkyl chain was elongated and decreased gradually when the alkyl carbon atoms exceeded 8. The fluorescence of erythrocyte membrane protein was quenched by Ber-C8-<i>n</i>, which showed a similar changing tendency on membrane fluidity. Experiments <i style="">in vitro</i> suggested that disturbing effects of Ber-C8-n on the conformation and function of membrane protein leaded to the changes of membrane fluidity and stability, and then the membrane was broken down.|
|ISSN:||0975-1009 (Online); 0019-5189 (Print)|
|Appears in Collections:||IJEB Vol.49(05) [May 2011]|
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