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|Title:||Isolation of a laccase with HIV-1 reverse transcriptase inhibitory activity from fresh fruiting bodies of the <i>Lentinus edodes</i> (Shiitake mushroom)<i></i>|
Ng, Tzi Bun
HIV-1 reverse transcriptase inhibitory activity
|Abstract:||A laccase with a molecular mass of 67 kDa and inhibitory activity toward HIV-1 reverse transcriptase (IC<sub>50</sub> = 7.5 <img src='/image/spc_char/micro.gif' border=0> M) was isolated from fresh fruiting bodies of the <i>Lentinus edodes</i> (Shiitake mushroom). Its characteristics were compared with those of laccases from cultured mushroom mycelia reported earlier. The laccase was unadsorbed on DEAE-cellulose, Affi-gel blue gel and CM-cellulose, but was adsorbed on Con A-Sepharose. About 50-fold purification was achieved with a 19.2% yield of the enzyme. The activity of the enzyme increased steadily from 20°C to 70°C. The activity disappeared after exposure to the boiling temperature for 10 min. Its optimal pH was 4 and very little enzyme activity remained at and above pH 10. The laccase inhibited HIV-1 reverse transcriptase with an IC<sub>50</sub> of 7.5 <img src='/image/spc_char/micro.gif' border=0> M, but did not demonstrate any antifungal or anti-proliferative activity.|
|ISSN:||0975-0959 (Online); 0301-1208 (Print)|
|Appears in Collections:||IJBB Vol.48(2) [April 2011]|
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