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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.48 [2011] >
IJBB Vol.48(2) [April 2011] >
| Title: | Isolation of a laccase with HIV-1 reverse transcriptase inhibitory activity from fresh fruiting bodies of the Lentinus edodes (Shiitake mushroom) |
| Authors: | Sun, Jian
Wang, Hexiang
Ng, Tzi Bun |
| Keywords: | Lentinus edodes
Shiitake mushroom
Laccase
HIV-1 reverse transcriptase inhibitory activity |
| Issue Date: | Apr-2011 |
| Publisher: | NISCAIR-CSIR, India |
| Abstract: | A laccase with a
molecular mass of 67 kDa and inhibitory activity toward HIV-1 reverse
transcriptase (IC50 = 7.5  M) was
isolated from fresh fruiting bodies of the Lentinus edodes (Shiitake
mushroom). Its
characteristics were compared with those of laccases from cultured mushroom
mycelia reported earlier. The laccase was unadsorbed on DEAE-cellulose,
Affi-gel blue gel and CM-cellulose, but was adsorbed on Con A-Sepharose. About 50-fold purification
was achieved with a 19.2% yield of
the enzyme. The activity of the enzyme increased steadily from 20°C to 70°C. The activity
disappeared after exposure to the boiling temperature for 10 min. Its optimal
pH was 4 and very little enzyme activity remained at and above pH 10. The
laccase inhibited HIV-1 reverse transcriptase with an IC50 of 7.5 M, but did not
demonstrate any antifungal or anti-proliferative activity. |
| Page(s): | 88-94 |
| CC License: |  CC Attribution-Noncommercial-No Derivative Works 2.5 India |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.48(2) [April 2011]
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