NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Journal of Scientific and Industrial Research (JSIR) >
JSIR Vol.66 [2007] >
JSIR Vol.66(02) [February 2007] >


Title: A validated stability indicating HPTLC method for determination of nitazoxanide
Authors: Gopu, C L
Thomas, Shibu
Paradkar, A R
Mahadik, K R
Keywords: HPTLC
ICH
Nitazoxanide
Stability-indicating method
Stress studies
Issue Date: Feb-2007
Publisher: CSIR
Abstract: A simple, selective, precise and stability-indicating high-performance thin-layer chromatographic (HPTLC) method for nitazoxanide in bulk drug and in formulations was developed and validated. Method employed TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. Solvent system consisted of ethyl acetate-toluene- methanol (4:6:1, v/v/v). Nitazoxanide was subjected to hydrolysis, oxidation, photolysis and thermal decomposition to establish a validated stability-indicating HPTLC method. Extensive degradation occurred in alkaline medium and in oxidative stress condition and degradation product was well separated from pure drug. Densitometric analysis of nitazoxanide was carried out in absorbance mode at 350 nm. Linear regression analysis data for mean calibration plots showed good linear relationship with r² = 0.9997 in 400-1600 ng per spot. Method was validated with respect to linearity, precision, accuracy, specificity and robustness. The limits of detection and quantitation were 15 and 50 ng per spot, respectively. HPTLC method could effectively separate drug from its degradation product and can be employed as a stability-indicating method.
Page(s): 141-145
ISSN: 0022–4456
Source:JSIR Vol.66(02) [February 2007]

Files in This Item:

File Description SizeFormat
JSIR 66(2) (2007) 141-145.pdf142.59 kBAdobe PDFView/Open
 Current Page Visits: 1173 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 621936 since 06-Feb-2009  Last updated on 13-Nov-2014Webmaster: nopr@niscair.res.in