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Title: Phytochemical analysis, antioxidant and xanthine oxidase inhibitory activity of <i style="">Tephrosia purpurea </i>Linn. root extract
Authors: Nile, Shivraj H
Khobragade, CN
Keywords: <i style="">Tephrosia purpurea</i>
Xanthine oxidase
Purple Tephrosia
Phytochemical analysis
Issue Date: Mar-2011
Publisher: NISCAIR-CSIR, India
Series/Report no.: <b style="">Int. cl.(2011.01)—</b>A61K 36/00, A61P 17/18
Abstract: An attempt has been made to search for xanthine oxidase (XO) inhibitors from the root extracts of <i style="">Tephrosia purpurea </i>Linn<i>. </i>which is<i> </i>traditionally used in folk medicine in India. Root extracts were screened for <i style="">in vitro</i> antioxidant and xanthine oxidase inhibitory activity. Antioxidant activity was measured using ABTS, DPPH, FRAP and ORAC methods. The enzyme inhibitory activity was tested on purified milk xanthine oxidase. The root extracts and phytochemicals, obtained in distilled water, inhibited bovine milk XO in a<i> </i>concentration-dependent manner, with an additional superoxide scavenging capacity, the average antioxidant activity of <i style="">T. purpurea</i> root extract in the concentration range of 100-200 <img src='/image/spc_char/micro.gif' border=0>g/mL. The reacting system revealed significant antioxidant activity, viz. 42.2 (ABTS), 28.7 (DPPH), 36.5 (FRAP) and 25.6 per cent by ORAC assay. Screening of xanthine oxidase inhibitory activity by extract in terms of kinetic parameters revealed noncompetitive mode of inhibition, where the K<sub>m</sub> and V<sub>max </sub>values in presence of (25 to 100 <img src='/image/spc_char/micro.gif' border=0>g/mL) <i style="">T. purpurea</i> root extract is 0.18 <img src='/image/spc_char/micro.gif' border=0>g and 0.040,0.037,0.034 and 0.030 (<img src='/image/spc_char/micro.gif' border=0>g/min) while for control K<sub>m</sub> and V<sub>max </sub>is 0.21 <img src='/image/spc_char/micro.gif' border=0>g and 0.043 (<img src='/image/spc_char/micro.gif' border=0>g/min), respectively. The phytochemical analysis revealed presence of significant amount of polyphenols and flavonoids (90% and 80%, respectively). These findings suggest that <i style="">T. purpurea </i>root extract possess prominent medicinal properties and can be exploited as natural drug to treat the diseases associated with free radical formation, oxidative stress and xanthine oxidase activity.
Description: 52-58
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Appears in Collections:IJNPR Vol.2(1) [March 2011]

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