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IJNPR Vol.2(1) [March 2011] >


Title: Antioxidative constitution of Mukia maderaspatana (Linn.) M. Roem. leaves
Authors: Petrus, A J A
Bhuvaneshwari, N
Alain, J A L
Keywords: Mukia maderaspatana
Cucurbitaceae
Antioxidant
Phytochemicals
Dietary source
Leafy vegetable
Saponarin.
Issue Date: Mar-2011
Publisher: NISCAIR-CSIR, India
IPC CodeInt. cl. (2011.01)—A61K 36/00, A61P 39/06.
Abstract: Indigenous leafy vegetables of the traditional rural communities are potentially nutritious and contain a vast array of beneficial phytochemicals that offer protection against several chronic and age-related human ailments. The present study was planned to assess the antioxidative constitution of the fresh leaves of Mukia maderaspatana (Linn.) M. Roem. (Family: Cucurbitaceae). The total phenolic, flavonoid, carotenoid and vitamin E contents were determined using specific spectrophotometric methods and the amount of ascorbic acid by titrimetry. The medicinal leafy vegetable was found to be a good dietary source of the low molecular weight antioxidants analysed. The leaf extract exhibited potent in vitro antioxidant/radical scavenging (ABTS•+ and FRAP assays) and metal (Ferrous ion) chelating activities and also inhibited β-carotene bleaching. Saponarin has been isolated and determined as the major phenolic antioxidant by HPLC. The amount of phenolics (292.392 ±1.087 mg GAE), flavonoid (247.079 ± 0.705 QE), carotenoids (0.812 ± 0.075 mg), vitamins C (17.046 ± 0.839 mg) and E (0.194 ± 0.068 mg) and saponarin (220.800 ± 0.944 mg) present and the antioxidant capacities (301.926 ± 0.869 mg vitamin C equivalent antioxidant capacity) are reported for 100 g of fresh leaves. The protective/therapeutic significance of these antioxidant components is also highlighted.
Page(s): 34-43
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-1033 (Online); 0379-5136 (Print)
Source:IJNPR Vol.2(1) [March 2011]

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