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IJBT Vol.10(2) [April 2011] >


Title: Exploration of Y-chromosome specific markers to discover SNP associated with sub fertility traits in dairy bulls
Authors: Mukhopadhyay, C S
Gupta, A K
Yadav, B R
Mohanty, T K
Keywords: Buffalo
Bulls Y-chromosome
SNP
Sub fertility
Issue Date: Apr-2011
Publisher: NISCAIR-CSIR, India
Abstract: The present study was designed to identify single nucleotide polymorphism (SNP) in Y-chromosome specific genes, namely, sex determining region, Y-encoded (SRY) and testis specific protein, Y-encoded (TSPY) vis-à-vis, detection of association between the SNP and andrological parameters (like, conception rate and per cent individual motility of semen). A total of 106 bulls, belonging to Karan Fries (Holstein Friesian crossbreds cattle), Sahiwal cattle and Murrah buffalo, were selected based on poor reproductive performance (viz., libido, semen quality & freezability), along with some control bulls with normal andrological parameters. Single strand conformation polymorphism followed by sequencing of the different band patterns revealed only a single novel SNP (Guanine to Adenine transition) in the 4th intron of TSPY gene in Murrah buffalo. There was no significant association between the genotypes and the andrological parameters under study. Further study, encompassing larger number of animals distributed over many farms, could reveal sizeable number of SNP and association with spermatological parameters.
Page(s): 178-182
CC License:  CC Attribution-Noncommercial-No Derivative Works 2.5 India
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.10(2) [April 2011]

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